Testosterone Physiology in Resistance Exercise and Training

The Up-Stream Regulatory Elements

Abstract

Testosterone is one of the most potent naturally secreted androgenicanabolic hormones, and its biological effects include promotion of muscle growth. In muscle, testosterone stimulates protein synthesis (anabolic effect) and inhibits protein degradation (anti-catabolic effect); combined, these effects account for the promotion of muscle hypertrophy by testosterone. These physiological signals from testosterone are modulated through the interaction of testosterone with the intracellular androgen receptor (AR). Testosterone is important for the desired adaptations to resistance exercise and training; in fact, testosterone is considered the major promoter of muscle growth and subsequent increase in muscle strength in response to resistance training in men. The acute endocrine response to a bout of heavy resistance exercise generally includes increased secretion of various catabolic (breakdown- related) and anabolic (growth-related) hormones including testosterone. The response of testosterone and AR to resistance exercise is largely determined by upper regulatory elements including the acute exercise programme variable domains, sex and age. In general, testosterone concentration is elevated directly following heavy resistance exercise in men. Findings on the testosterone response in women are equivocal with both increases and no changes observed in response to a bout of heavy resistance exercise. Age also significantly affects circulating testosterone concentrations. Until puberty, children do not experience an acute increase in testosterone from a bout of resistance exercise; after puberty some acute increases in testosterone from resistance exercise can be found in boys but not in girls. Aging beyond 35–40 years is associated with a 1–3% decline per year in circulating testosterone concentration in men; this decline eventually results in the condition known as andropause. Similarly, aging results in a reduced acute testosterone response to resistance exercise in men. In women, circulating testosterone concentration also gradually declines until menopause, after which a drastic reduction is found. In summary, testosterone is an important modulator of muscle mass in both men and women and acute increases in testosterone can be induced by resistance exercise. In general, the variables within the acute programme variable domains must be selected such that the resistance exercise session contains high volume and metabolic demand in order to induce an acute testosterone response.

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References

  1. 1.

    Mendelson C, Dufau M, Catt K. Gonadotropin binding and stimulation of cyclic adenosine 30:50-monophosphateand testosterone production in isolated Leydig cells. J Biol Chem 1975 Nov 25; 250 (22): 8818–23

    PubMed  CAS  Google Scholar 

  2. 2.

    Liedman R, Hansson SR, Howe D, et al. Reproductive hormones in plasma over the menstrual cycle in primarydysmenorrhea compared with healthy subjects. Gynecol Endocrinol 2008 Sep; 24 (9): 508–13

    PubMed  CAS  Article  Google Scholar 

  3. 3.

    Dehennin L, Bonnaire Y, Plou P. Human nutritional supplements in the horse. Dehydroepiandrosterone versusandrostenedione: comparative effects on the androgenprofile and consequences for doping analysis J AnalToxicol 2001 Nov-Dec; 25 (8): 685–90

    CAS  Google Scholar 

  4. 4.

    Marouliss GB, Triantafillidis IK. Polycystic ovarian disease: the adrenal connection. Pediatr Endocrinol Rev2006 Jan; Suppl.; 1: 205–7

  5. 5.

    Conley AJ, Bird IM. The role of cytochrome P450 17 alpha- hydroxylase and 3 beta-hydroxysteroid dehydrogenasein the integration of gonadal and adrenal steroidogenesisvia the delta 5 and delta 4 pathways ofsteroidogenesis in mammals. Biol Reprod 1997 Apr; 56 (4): 789–99

    PubMed  CAS  Article  Google Scholar 

  6. 6.

    Labrie F. Intracrinology: molecular and cellular endocrinology 1991 Jul; 78 (3): C113–8

    Article  Google Scholar 

  7. 7.

    Vingren JL, Kraemer WJ, Hatfield DL, et al. Effect of resistance exercise on muscle steroidogenesis. J Appl Physiol 2008 Dec; 105 (6): 1754–60

    PubMed  Article  Google Scholar 

  8. 8.

    Kim HH. Regulation of gonadotropin-releasing hormone gene expression. Semin Reprod Med 2007 Sep; 25 (5): 313–25

    PubMed  CAS  Article  Google Scholar 

  9. 9.

    Miller WL. Molecular biology of steroid hormone synthesis. Endocr Rev 1988 Aug; 9 (3): 295–318

    PubMed  CAS  Article  Google Scholar 

  10. 10.

    Payne AH. Hormonal regulation of cytochrome P450 enzymes, cholesterol side-chain cleavage and 17 alpha-hydroxylase/C17-20 lyase in Leydig cells. Biol Reprod 1990 Mar; 42 (3): 399–404

    PubMed  CAS  Article  Google Scholar 

  11. 11.

    Kvorning T, Andersen M, Brixen K, et al. Suppression of endogenous testosterone production attenuates the responseto strength training: a randomized, placebo-controlled,and blinded intervention study. Am J Physiol 2006 Dec; 291 (6): E1325–32

    CAS  Google Scholar 

  12. 12.

    Kvorning T, Andersen M, Brixen K, et al. Suppression of testosterone does not blunt mRNA expression of myoD,myogenin, IGF, myostatin or androgen receptor poststrength training in humans. J Physiol 2007 Jan 15; 578 (Pt2): 579–93

    PubMed  CAS  Google Scholar 

  13. 13.

    Florini J. Effects of testosterone on qualitative pattern of protein synthesis in skeletal muscle. Biochemistry 9 (4): 909–12

  14. 14.

    Wilson JD, Foster DW, Kronenberg HM, et al., editors. Williams textbook of endocrinology. 9th ed. Philadelphia(PA): W.B. Saunders Company, 1998

    Google Scholar 

  15. 15.

    Mauras N, Hayes V, Welch S, et al. Testosterone deficiency in young men: marked alterations in whole body proteinkinetics, strength, and adiposity. J Clin Endocrinol Metab 1998 Jun; 83 (6): 1886–92

    PubMed  CAS  Article  Google Scholar 

  16. 16.

    Demling RH, Orgill DP. The anticatabolic and wound healing effects of the testosterone analog oxandroloneafter severe burn injury. J Crit Care 2000 Mar; 15 (1): 12–7

    PubMed  CAS  Article  Google Scholar 

  17. 17.

    Gobinet J, Poujol N, Sultan C. Molecular action of androgens. Mol Cell Endocrinol 2002 Dec 30; 198 (1-2): 15–24

    PubMed  CAS  Article  Google Scholar 

  18. 18.

    Falkenstein E, Tillmann HC, Christ M, et al. Multiple actions of steroid hormones: a focus on rapid, nongenomiceffects. Pharm Rev 2000 Dec; 52 (4): 513–56

    PubMed  CAS  Google Scholar 

  19. 19.

    Mayer M, Rosen F. Interaction of anabolic steroids with glucocorticoid receptor sites in rat muscle cytosol. Am JPhysiol 1975 Nov; 229 (5): 1381–6

    CAS  Google Scholar 

  20. 20.

    Mayer M, Rosen F. Interaction of glucocorticoids and androgens with skeletal muscle. Metab Clin Exper 26 (8): 937–62

  21. 21.

    Crawford BA, Liu PY, Kean MT, et al. Randomized placebo-controlled trial of androgen effects on muscleand bone in men requiring long-term systemic glucocorticoidtreatment. J Clin Endocrinol Metab 2003 Jul; 88 (7): 3167–76

    PubMed  CAS  Article  Google Scholar 

  22. 22.

    Hiraoka D, Nakamura N, Nishizawa Y, et al. Inhibitory and stimulatory effects of glucocorticoid on androgeninducedgrowth of murine Shionogi carcinoma 115 in vivoand in cell culture. Cancer Res 1987 Dec 15; 47 (24Pt1): 6560–4

    PubMed  CAS  Google Scholar 

  23. 23.

    Hardy MP, Gao HB, Dong Q, et al. Stress hormone and male reproductive function. Cell Tissue Res 2005 Oct; 322 (1): 147–53

    PubMed  CAS  Article  Google Scholar 

  24. 24.

    Schwarz S, Pohl P. Steroid hormones and steroid hormone binding globulins in cerebrospinal fluid studied in individualswith intact and with disturbed blood-cerebrospinalfluid barrier. Neuroendocrinology 1992 Feb; 55 (2): 174–82

    PubMed  CAS  Article  Google Scholar 

  25. 25.

    Cumming DC, Wall SR. Non-sex hormone-binding globulinbound testosterone as a marker for hyperandrogenism. J Clin Endocrinol Metab 1985 Nov; 61 (5): 873–6

    PubMed  CAS  Article  Google Scholar 

  26. 26.

    Dunn JF, Nisula BC, Rodbard D. Transport of steroid hormones: binding of 21 endogenous steroids to bothtestosterone-binding globulin and corticosteroid-bindingglobulin in human plasma. J Clin Endocrinol Metab 53 (1): 58–68

  27. 27.

    Kraemer WJ, Hakkinen K, Newton RU, et al. Acute hormonal responses to heavy resistance exercise in youngerand older men. Eur J Applied Physiol Occup Physiol 1998 Feb; 77 (3): 206–11

    CAS  Article  Google Scholar 

  28. 28.

    Jeyaraj DA, Grossman G, Petrusz P. Altered bioavailability of testosterone in androgen-binding protein-transgenicmice. Steroids 2005 Sep; 70 (10): 704–14

    PubMed  CAS  Article  Google Scholar 

  29. 29.

    Hobbs CJ, Jones RE, Plymate SR. The effects of sex hormone binding globulin (SHBG) on testosterone transportinto the cerebrospinal fluid. J Steroid Biochem Mol Biol 1992 Jul; 42 (6): 629–35

    PubMed  CAS  Article  Google Scholar 

  30. 30.

    Pardridge WM, Mietus LJ. Transport of steroid hormones through the rat blood-brain barrier: primary role of albumin-bound hormone. J Clin Invest 1979 Jul; 64 (1): 145–54

    PubMed  CAS  Article  Google Scholar 

  31. 31.

    Manni A, Pardridge WM, Cefalu W, et al. Bioavailability of albumin-bound testosterone. J Clin Endocrinol Metab 1985 Oct; 61 (4): 705–10

    PubMed  CAS  Article  Google Scholar 

  32. 32.

    Baulieu E, Robel P. Catabolism of testosterone and androstenedione. In: Eik-Nes K, editor. The androgens ofthe testis. New York (NY): Marcel Dekker Inc., 1970: 50–70

    Google Scholar 

  33. 33.

    Ferrando AA, Sheffield-Moore M, Yeckel CW, et al. Testosterone administration to older men improves musclefunction: molecular and physiological mechanisms. Am JPhysiol 2002 Mar; 282 (3): E601–7

    CAS  Google Scholar 

  34. 34.

    Kadi F, Eriksson A, Holmner S, et al. Effects of anabolic steroids on the muscle cells of strength-trained athletes. Med Sci Sports Exerc 1999 Nov; 31 (11): 1528–34

    PubMed  CAS  Article  Google Scholar 

  35. 35.

    Lee WJ, McClung J, Hand GA, et al. Overload-induced androgen receptor expression in the aged rat hind limbreceiving nandrolone decanoate. J Appl Physiol 2003 Mar1; 94 (3): 1153–61

    PubMed  CAS  Google Scholar 

  36. 36.

    Carson JA, Lee WJ, McClung J, et al. Steroid receptor concentration in aged rat hind limb muscle: effect ofanabolic steroid administration. J Appl Physiol 2002 Jul; 93 (1): 242–50

    PubMed  CAS  Google Scholar 

  37. 37.

    Syms AJ, Norris JS, Panko WB, et al. Mechanism of androgen- receptor augmentation: analysis of receptorsynthesis and degradation by the density-shift technique. J Biol Chem 1985 Jan 10; 260 (1): 455–61

    PubMed  CAS  Google Scholar 

  38. 38.

    Gregory CW, Johnson Jr RT, Mohler JL, et al. Androgen receptor stabilization in recurrent prostate cancer isassociated with hypersensitivity to low androgen. Cancer Res 2001 Apr 1; 61 (7): 2892–8

    PubMed  CAS  Google Scholar 

  39. 39.

    Sinha-Hikim I, Taylor WE, Gonzalez-Cadavid NF, et al. Androgen receptor in human skeletal muscle and culturedmuscle satellite cells: up-regulation by androgen treatment. J Clin Endocrinol Metab 2004 Oct; 89 (10): 5245–55

    PubMed  CAS  Article  Google Scholar 

  40. 40.

    Richmond EJ, Rogol AD. Male pubertal development and the role of androgen therapy. Nature Clin Pract 2007 Apr; 3 (4): 338–44

    CAS  Article  Google Scholar 

  41. 41.

    Kaufman JM, Vermeulen A. Declining gonadal function in elderly men. Bailliere’s Clin Endocrinol Metab 1997 Jul; 11 (2): 289–309

    CAS  Article  Google Scholar 

  42. 42.

    Stearns EL, MacDonnell JA, Kaufman BJ, et al. Declining testicular function with age: hormonal and clinical correlates. Am J Med 1974 Nov; 57 (5): 761–6

    PubMed  CAS  Article  Google Scholar 

  43. 43.

    Tenover JS. Declining testicular function in aging men. Int J Impotence Res 2003 Aug; 15 Suppl.4: S3–8

    Article  CAS  Google Scholar 

  44. 44.

    Kumar RJ, Barqawi A, Crawford ED. Adverse events associated with hormonal therapy for prostate cancer. Rev Urol 2005; 7 Suppl.5: S37–43

    Google Scholar 

  45. 45.

    Axell AM, MacLean HE, Plant DR, et al. Continuous testosterone administration prevents skeletal muscleatrophy and enhances resistance to fatigue in orchidectomizedmale mice. Am J Physiol 2006 Sep; 291 (3): E506–16

    CAS  Google Scholar 

  46. 46.

    Galvao DA, Nosaka K, Taaffe DR, et al. Resistance training and reduction of treatment side effects in prostatecancer patients. Med Sci Sports Exerc 2006 Dec; 38 (12): 2045–52

    PubMed  Article  Google Scholar 

  47. 47.

    Chakravarti S, Collins WP, Forecast JD, et al. Hormonal profiles after the menopause. BMJ 1976 Oct 2; 2 (6039): 784–7

    PubMed  CAS  Article  Google Scholar 

  48. 48.

    Miller KK, Biller BM, Beauregard C, et al. Effects of testosterone replacement in androgen-deficient women withhypopituitarism: a randomized, double-blind, placebocontrolledstudy. J Clin Endocrinol Metab 2006 May; 91 (5): 1683–90

    PubMed  CAS  Article  Google Scholar 

  49. 49.

    Floter A, Nathorst-Boos J, Carlstrom K, et al. Effects of combined estrogen/testosterone therapy on bone andbody composition in oophorectomized women. Gynecol Endocrinol 2005 Mar; 20 (3): 155–60

    PubMed  CAS  Article  Google Scholar 

  50. 50.

    Mauras N, Haymond MW, Darmaun D, et al. Calcium and protein kinetics in prepubertal boys: positive effects oftestosterone. J Clin Investig 1994 Mar; 93 (3): 1014–9

    PubMed  CAS  Article  Google Scholar 

  51. 51.

    Mauras N, Rini A, Welch S, et al. Synergistic effects of testosterone and growth hormone on protein metabolismand body composition in prepubertal boys. Metab Clin Exper 2003 Aug; 52 (8): 964–9

    CAS  Article  Google Scholar 

  52. 52.

    Wu Y, Zhao W, Zhao J, et al. Identification of androgen response elements in the insulin-like growth factor I upstreampromoter. Endocrinology 2007 Jun; 148 (6): 2984–93

    PubMed  CAS  Article  Google Scholar 

  53. 53.

    Glass DJ. Skeletal muscle hypertrophy and atrophy signaling pathways. Int J Biochem Cell Biol 2005 Oct; 37 (10): 1974–84

    PubMed  CAS  Article  Google Scholar 

  54. 54.

    Nindl BC, Kraemer WJ, Gotshalk LA, et al. Testosterone responses after resistance exercise in women: influence ofregional fat distribution. Int J Sport Nutr Exerc Metab 2001 Dec; 11 (4): 451–65

    PubMed  CAS  Google Scholar 

  55. 55.

    Copeland JL, Consitt LA, Tremblay MS. Hormonal responses to endurance and resistance exercise in femalesaged 19-69 years. J Gerontol 2002 Apr; 57 (4): B158–65

    Article  Google Scholar 

  56. 56.

    Linnamo V, Pakarinen A, Komi PV, et al. Acute hormonal responses to submaximal and maximal heavy resistanceand explosive exercises in men and women. J Strength Cond Res 2005 Aug; 19 (3): 566–71

    PubMed  Google Scholar 

  57. 57.

    Consitt LA, Copeland JL, Tremblay MS. Hormone responses to resistance vs endurance exercise in premenopausalfemales. Can J Appl Physiol 2001 Dec; 26 (6): 574–87

    PubMed  CAS  Article  Google Scholar 

  58. 58.

    Kraemer WJ, Fleck SJ, Dziados JE, et al. Changes in hormonal concentrations after different heavy-resistance exerciseprotocols in women. J Appl Physiol 1993 Aug; 75 (2): 594–604

    PubMed  CAS  Google Scholar 

  59. 59.

    Hakkinen K, Pakarinen A. Acute hormonal responses to two different fatiguing heavy-resistance protocols in maleathletes. J Appl Physiol 1993 Feb; 74 (2): 882–7

    PubMed  CAS  Google Scholar 

  60. 60.

    Koziris LP, Kraemer WJ, Gordon SE, et al. Effect of acute postexercise ethanol intoxication on the neuroendocrine response to resistance exercise. J Appl Physiol 2000 Jan; 88 (1): 165–72

    PubMed  CAS  Google Scholar 

  61. 61.

    Kraemer WJ, Marchitelli L, Gordon SE, et al. Hormonal and growth factor responses to heavy resistance exerciseprotocols. J Appl Physiol 1990 Oct; 69 (4): 1442–50

    PubMed  CAS  Google Scholar 

  62. 62.

    Hakkinen K, Pakarinen A, Kraemer WJ, et al. Basal concentrations and acute responses of serum hormones andstrength development during heavy resistance training inmiddle-aged and elderly men and women. J Gerontol 2000 Feb; 55 (2): B95–105

    Article  Google Scholar 

  63. 63.

    Pullinen T, Mero A, Huttunen P, et al. Resistance exerciseinduced hormonal responses in men, women, and pubescentboys. Med Sci Sports Exerc 2002 May; 34 (5): 806–13

    PubMed  CAS  Article  Google Scholar 

  64. 64.

    Smilios I, Pilianidis T, Karamouzis M, et al. Hormonal responses after various resistance exercise protocols. Med Sci Sports Exerc 2003 Apr 1; 35 (4): 644–54

    PubMed  CAS  Article  Google Scholar 

  65. 65.

    Willoughby D, Taylor L. Effects of sequential bouts of resistance exercise on androgen receptor expression. Med Sci Sports Exerc 2004 Sep 1; 36 (9): 1499–506

    PubMed  CAS  Article  Google Scholar 

  66. 66.

    Ratamess NA, Kraemer WJ, Volek JS, et al. Androgen receptor content following heavy resistance exercise inmen. J Steroid Biochem Mol Biol 2005 Jan; 93 (1): 35–42

    PubMed  CAS  Article  Google Scholar 

  67. 67.

    Kraemer WJ, Spiering BA, Volek JS, et al. Androgenic responses to resistance exercise: effects of feeding andL-carnitine. Med Sci Sports Exerc 2006 Jul; 38 (7): 1288–96

    PubMed  CAS  Article  Google Scholar 

  68. 68.

    Yarrow JF, Borsa PA, Borst SE, et al. Neuroendocrine responses to an acute bout of eccentric-enhanced resistanceexercise. Med Sci Sports Exerc 2007 Jun; 39 (6): 941–7

    PubMed  CAS  Google Scholar 

  69. 69.

    Raastad T, Bjoro T, Hallen J. Hormonal responses to highand moderate-intensity strength exercise. Eur J Appl Physiol 2000 May; 82 (1-2): 121–8

    PubMed  CAS  Article  Google Scholar 

  70. 70.

    Fry AC, Kraemer WJ, Ramsey LT. Pituitary-adrenalgonadal responses to high-intensity resistance exerciseovertraining. J Appl Physiol 1998 Dec; 85 (6): 2352–9

    PubMed  CAS  Google Scholar 

  71. 71.

    Goto K, Ishii N, Kizuka T, et al. The impact of metabolic stress on hormonal responses and muscular adaptations. Med Sci Sports Exerc 2005 Jun; 37 (6): 955–63

    PubMed  CAS  Google Scholar 

  72. 72.

    Izquierdo M, Ibanez J, Gonzalez-Badillo JJ, et al. Differential effects of strength training leading to failure versus notto failure on hormonal responses, strength, and musclepower gains. J Appl Physiol 2006 May; 100 (5): 1647–56

    PubMed  CAS  Article  Google Scholar 

  73. 73.

    Migiano MJ, Vingren JL, Volek JS, et al. Endocrine response patterns to acute unilateral and bilateral resistanceexercise in men. J Strength Cond Res 2010 Jan; 24 (1): 128–34

    PubMed  Article  Google Scholar 

  74. 74.

    Spiering BA, Kraemer WJ, Vingren JL, et al. Elevated endogenous testosterone concentrations potentiate muscleandrogen receptor responses to resistance exercise. J Steroid Biochem Mol Biol 2009 Apr; 114 (3-5): 195–9

    PubMed  CAS  Article  Google Scholar 

  75. 75.

    Wilkinson SB, Tarnopolsky MA, Grant EJ, et al. Hypertrophy with unilateral resistance exercise occurs withoutincreases in endogenous anabolic hormone concentration. Eur J Appl Physiol 2006 Dec; 98 (6): 546–55

    PubMed  CAS  Article  Google Scholar 

  76. 76.

    Hansen S, Kvorning T, Kjaer M, et al. The effect of shortterm strength training on human skeletal muscle: the importanceof physiologically elevated hormone levels. Scand J Med Sci Sports 2001 Dec 1; 11 (6): 347–54

    PubMed  CAS  Article  Google Scholar 

  77. 77.

    Volek JS, Kraemer WJ, Bush JA, et al. Testosterone and cortisol in relationship to dietary nutrients and resistanceexercise. J Appl Physiol 1997 Jan; 82 (1): 49–54

    PubMed  CAS  Article  Google Scholar 

  78. 78.

    Kraemer WJ, Hakkinen K, Newton RU, et al. Effects of heavy-resistance training on hormonal response patternsin younger vs older men. J Appl Physiol 1999 Sep; 87 (3): 982–92

    PubMed  CAS  Google Scholar 

  79. 79.

    Kraemer WJ, Fry AC, Warren BJ, et al. Acute hormonal responses in elite junior weightlifters. Int J Sports Med 1992 Feb; 13 (2): 103–9

    PubMed  CAS  Article  Google Scholar 

  80. 80.

    Reeves GV, Kraemer RR, Hollander DB, et al. Comparison of hormone responses following light resistance exercisewith partial vascular occlusion and moderately difficultresistance exercise without occlusion. J Appl Physiol 2006 Dec; 101 (6): 1616–22

    PubMed  CAS  Article  Google Scholar 

  81. 81.

    Hakkinen K, Pakarinen A, Newton RU, et al. Acute hormone responses to heavy resistance lower and upper extremityexercise in young versus old men. Eur J Appl Physiol Occup Physiol 1998 Mar; 77 (4): 312–9

    PubMed  CAS  Article  Google Scholar 

  82. 82.

    Durand RJ, Castracane VD, Hollander DB, et al. Hormonal responses from concentric and eccentric musclecontractions. Med Sci Sports Exerc 2003 Jun; 35 (6): 937–43

    PubMed  CAS  Article  Google Scholar 

  83. 83.

    Kraemer RR, Hollander DB, Reeves GV, et al. Similar hormonal responses to concentric and eccentric muscleactions using relative loading. Eur J Appl Physiol 2006 Mar; 96 (5): 551–7

    Article  CAS  Google Scholar 

  84. 84.

    Krylow AM, Sandercock TG. Dynamic force responses of muscle involving eccentric contraction. J Biomech 30 (1): 27–33

  85. 85.

    Erskine J, Smillie I, Leiper J, et al. Neuromuscular and hormonal responses to a single session of whole body vibrationexercise in healthy young men. Clin Physiol Funct Imaging 2007 Jul; 27 (4): 242–8

    PubMed  Article  Google Scholar 

  86. 86.

    Kvorning T, Bagger M, Caserotti P, et al. Effects of vibration and resistance training on neuro muscular and hormonal measures. Eur J Applied Physiol 2006 Mar; 96 (5): 615–25

    CAS  Article  Google Scholar 

  87. 87.

    Di Loreto C, Ranchelli A, Lucidi P, et al. Effects of wholebody vibration exercise on the endocrine system of healthymen. J Endocrinol Invest 2004 Apr; 27 (4): 323–7

    PubMed  Google Scholar 

  88. 88.

    Bosco C, Iacovelli M, Tsarpela O, et al. Hormonal responses to whole-body vibration in men. Eur J Appl Physiol 2000 Apr; 81 (6): 449–54

    PubMed  CAS  Article  Google Scholar 

  89. 89.

    Spreuwenberg LP, Kraemer WJ, Spiering BA, et al. Influence of exercise order in a resistance-training exercisesession. J Strength Cond Res 2006 Feb; 20 (1): 141–4

    PubMed  Google Scholar 

  90. 90.

    Ratamess NA, Falvo MJ, Mangine GT, et al. The effect of rest interval length on metabolic responses to the benchpress exercise. Eur J Appl Physiol 2007 May; 100 (1): 1–17

    PubMed  Article  Google Scholar 

  91. 91.

    Ahtiainen JP, Pakarinen A, Alen M, et al. Short vs long rest period between the sets in hypertrophic resistance training:influence on muscle strength, size, and hormonaladaptations in trained men. J Strength Cond Res 2005 Aug; 19 (3): 572–82

    PubMed  Google Scholar 

  92. 92.

    Vingren JL, Kraemer WJ, Hatfield DL, et al. Effect of resistance exercise on muscle steroid receptor protein contentin strength-trained men and women. Steroids 2009 Nov-Dec; 74 (13-14): 1033–9

    PubMed  CAS  Article  Google Scholar 

  93. 93.

    Marx JO, Ratamess NA, Nindl BC, et al. Low-Vol. circuit versus high-Vol. periodized resistance trainingin women. Med Sci Sports Exerc 2001 Apr; 33 (4): 635–43

    PubMed  CAS  Google Scholar 

  94. 94.

    Nakamura Y, Hornsby PJ, Casson P, et al. Type 5 17betahydroxysteroid dehydrogenase (AKR1C3) contributes totestosterone production in the adrenal reticularis. J Clin Endocrinol Metab 2009 Jun; 94 (6): 2192–8

    PubMed  CAS  Article  Google Scholar 

  95. 95.

    Kraemer WJ, French DN, Spiering BA, et al. Cortitrol supplementation reduces serum cortisol responses tophysical stress. Metab Clin Exper 2005 May; 54 (5): 657–68

    CAS  Article  Google Scholar 

  96. 96.

    Korth-Schutz S, Levine LS, New MI. Serum androgens in normal prepubertal and pubertal children and in childrenwith precocious adrenarche. J Clin Endocrinol Metab 1976; 42 (1): 117–24

    PubMed  CAS  Article  Google Scholar 

  97. 97.

    Feldman HA, Longcope C, Derby CA, et al. Age trends in the level of serum testosterone and other hormones inmiddle-aged men: longitudinal results from the Massachusettsmale aging study. J Clin Endocrinol Metab 2002 Feb; 87 (2): 589–98

    PubMed  CAS  Article  Google Scholar 

  98. 98.

    Vermeulen A, Deslypere JP, De Meirleir K. A new look to the andropause: altered function of the gonadotrophs. J Steroid Biochem 1989 Jan; 32 (1B): 163–5

    PubMed  CAS  Article  Google Scholar 

  99. 99.

    Pullinen T, Mero A, MacDonald E, et al. Plasma catecholamine and serum testosterone responses to four units ofresistance exercise in young and adult male athletes. Eur JAppl Physiol Occup Physiol 1998 Apr; 77 (5): 413–20

    CAS  Article  Google Scholar 

  100. 100.

    Fahey TD, Rolph R, Moungmee P, et al. Serum testosterone, body composition, and strength of young adults. Med Sci Sports 1976 Spring; 8 (1): 31–4

    PubMed  CAS  Google Scholar 

  101. 101.

    Baker JR, Anderson MA, et al. Effects of age on testosterone responses to resistance exercise and musculoskeletalvariables in men. J Strength Cond Res 2006 Nov; 20 (4): 874–81

    PubMed  Google Scholar 

  102. 102.

    Hayashi T, Yamada T. Association of bioavailable estradiol levels and testosterone levels with serum albumin levelsin elderly men. Aging Male 2008 Jun; 11 (2): 63–70

    PubMed  CAS  Article  Google Scholar 

  103. 103.

    Rodriguez A, Muller DC, Metter EJ, et al. Aging, androgens, and the metabolic syndrome in a longitudinal studyof aging. J Clin Endocrinol Metab 2007 Sep; 92 (9): 3568–72

    PubMed  CAS  Article  Google Scholar 

  104. 104.

    Hakkinen K, Pakarinen A, Kraemer WJ, et al. Selective muscle hypertrophy, changes in EMG and force, andserum hormones during strength training in older women. J Appl Physiol 2001 Aug; 91 (2): 569–80

    PubMed  CAS  Google Scholar 

  105. 105.

    Borst SE, Vincent KR, Lowenthal DT, et al. Effects of resistance training on insulin-like growth factor and itsbinding proteins in men and women aged 60 to 85. J Am Geriatr Soc 2002 May; 50 (5): 884–8

    PubMed  Article  Google Scholar 

  106. 106.

    Petrella JK, Kim JS, Cross JM, et al. Efficacy of myonuclear addition may explain differential myofiber growthamong resistance-trained young and older men andwomen. Am J Physiol 2006 Nov; 291 (5): E937–46

    CAS  Google Scholar 

  107. 107.

    Daly RM, Dunstan DW, Owen N, et al. Does highintensity resistance training maintain bone mass duringmoderate weight loss in older overweight adults with type2 diabetes? Osteoporos Int 2005 Dec; 16 (12): 1703–12

    PubMed  Article  Google Scholar 

  108. 108.

    Bamman MM, Shipp JR, Jiang J, et al. Mechanical load increases muscle IGF-I and androgen receptor mRNA concentrations in humans. Am J Physiol 2001 Mar 1; 280 (3): E383–90

    CAS  Google Scholar 

  109. 109.

    Lee WJ, Thompson RW, McClung JM, et al. Regulation of androgen receptor expression at the onset of functionaloverload in rat plantaris muscle. Am J Physiol Regulatory Integrative Comp Physiol 2003 Nov 1; 285 (5): R1076–85

    CAS  Google Scholar 

  110. 110.

    Inoue K, Yamasaki S, Fushiki T, et al. Rapid increase in the number of androgen receptors following electricalstimulation of the rat muscle. Eur J Appl Physiol OccupPhysiol 1993 Jan 1; 66 (2): 134–40

    PubMed  CAS  Article  Google Scholar 

  111. 111.

    Tchaikovsky VS, Astratenkova JV, Basharina OB. The effect of exercises on the content and reception of thesteroid hormones in rat skeletal muscles. J Steroid Biochem 1986 Jan; 24 (1): 251–3

    PubMed  CAS  Article  Google Scholar 

  112. 112.

    Inoue K, Yamasaki S, Fushiki T, et al. Androgen receptor antagonist suppresses exercise-induced hypertrophy ofskeletal muscle. Eur J Appl Physiol Occup Physiol 1994 Jan 1; 69 (1): 88–91

    PubMed  CAS  Article  Google Scholar 

  113. 113.

    Kraemer WJ, Volek JS, Bush JA, et al. Hormonal responses to consecutive days of heavy-resistance exercisewith or without nutritional supplementation. J Appl Physiol 1998 Oct; 85 (4): 1544–55

    PubMed  CAS  Google Scholar 

  114. 114.

    Chandler RM, Byrne HK, Patterson JG, et al. Dietary supplements affect the anabolic hormones after weighttrainingexercise. J Appl Physiol 1994 Feb; 76 (2): 839–45

    PubMed  CAS  Google Scholar 

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Acknowledgements

No sources of funding were used to assist in the preparation of this review. The authors have no potential conflicts of interest that are directly relevant to the content of this review.

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Correspondence to Dr William J. Kraemer.

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Vingren, J.L., Kraemer, W.J., Ratamess, N.A. et al. Testosterone Physiology in Resistance Exercise and Training. Sports Med 40, 1037–1053 (2010). https://doi.org/10.2165/11536910-000000000-00000

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Keywords

  • Testosterone
  • Androgen Receptor
  • Resistance Training
  • Resistance Exercise
  • Leydig Cell