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MultiPrimer

Software for Multiplex Primer Design

  • Biomedical Genomics and Proteomics
  • Published:
Applied Bioinformatics

Abstract

This study presents a novel application, MultiPrimer, to assist users to design multiplex PCR primers. MultiPrimer adopts an algorithm based on a genetic algorithm and can efficiently solve the multi-objective optimisation problem. A match pattern model is proposed to speed up examination of the specificity constraint. MultiPrimer is able to find a set of primer pairs simultaneously for a set of gene family sequences and multiple targets for a single cDNA sequence. A public version of the MultiPrimer software is available from http://edith.cse.nsysu.edu.tw/new/software/MultiPrimer.htm.

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Acknowledgements

We thank Mr Chien-Chang Wu for valuable disucussions. We also thank for Mr Yuan-Ping Lin for performing the PCR experiments.

The authors have no conflicts of interest that are directly relevant to the content of this article.

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Authors and Affiliations

  1. Department of Computer Science and Engineering, National Sun Yat-Sen University, No. 70, Lienhai Road, Kaohsiung, 804, Taiwan

    Chungnan Lee, Jain-Shing Wu & Hong-Long Liang

  2. Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan

    Yow-Ling Shiue

Authors
  1. Chungnan Lee
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  2. Jain-Shing Wu
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  3. Yow-Ling Shiue
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  4. Hong-Long Liang
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Correspondence to Chungnan Lee.

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Lee, C., Wu, JS., Shiue, YL. et al. MultiPrimer. Appl-Bioinformatics 5, 99–109 (2006). https://doi.org/10.2165/00822942-200605020-00004

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  • DOI: https://doi.org/10.2165/00822942-200605020-00004

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