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Effect of the Sulphonylurea Glibenclamide on Liver and Kidney Antioxidant Enzymes in Streptozocin-Induced Diabetic Rats

Drugs in R & D volume 5, pages 203–208 (2004)Cite this article

Abstract

Background: Alterations in catalase (CAT) and superoxide dismutase (SOD) activity have been reported in diabetes mellitus. Glibenclamide (glyburide), a member of the second-generation sulphonylureas, provides effective treatment for patients with moderate diabetes. The action of the liver plays an important role in its glucose-lowering effect, suggesting that glibenclamide also exerts a direct effect on liver enzyme activities.

Objective: To evaluate the effect of glibenclamide on the activities of antioxidant enzymes (CAT and SOD) in liver and kidney tissue of diabetic rats.

Methods: Thirty-nine rats were included in this study. Moderate diabetes mellitus was induced with streptozocin (freshly dissolved in citrate buffer, ph 4.5) 55 mg/ kg in 22 rats. Eight of these diabetic rats were left untreated, insulin was administered to six diabetic rats, and glibenclamide was administered to eight rats with moderate diabetes. Liver and kidney CAT and SOD activities were measured in all rats.

Results: Hepatic CAT and SOD activities were significantly reduced in diabetic animals (p < 0.05 for both activities). Glibenclamide treatment of diabetic rats for 5 weeks reversed the changes observed in diabetic liver tissues (p < 0.05). However, renal CAT and SOD activities were unchanged. In addition, high blood glucose levels of diabetic rats were decreased following glibenclamide treatment.

Conclusion: Administration of glibenclamide to diabetic rats reversed diabetes-induced changes, suggesting that glibenclamide may directly increase liver CAT and SOD activity.

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Acknowledgements

This study was supported by the Gazi University Research Foundation. The authors have provided no information on sources of funding or on conflicts of interest directly relevant to the content of this study.

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  1. Department of Biochemisty, Gazi University, Medical Faculty, Beşevler, Ankara, 06510, Turkey

    Esra Elmalí &  Nilgün Altan

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  1. Esra Elmalí
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  2. Nilgün Altan
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  3. Neslihan Bukan
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Correspondence to Nilgün Altan.

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Elmalí, E., Altan, N. & Bukan, N. Effect of the Sulphonylurea Glibenclamide on Liver and Kidney Antioxidant Enzymes in Streptozocin-Induced Diabetic Rats. Drugs R D 5, 203–208 (2004). https://doi.org/10.2165/00126839-200405040-00003

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  • DOI: https://doi.org/10.2165/00126839-200405040-00003

Keywords

  • Glibenclamide
  • Sulphonylurea
  • Glipizide
  • Moderate Diabetes
  • Streptozocin