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Molecular Analysis of the P27/Kip1 Gene in Breast Cancer

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Abstract

Background: Genetic polymorphisms and mutations of the genes involved in tumorigenesis may determine individual susceptibility for cancer. The p27/Kip1 protein belongs to the family of cyclin-dependent kinase-inhibitory proteins, which are negative regulators of cell-cycle progression. Reduced protein levels of p27/Kip1 have been reported in numerous human cancers including breast cancer.

Methods and results: p27 gene mutations and the codon 109 polymorphism were investigated in breast cancer patients by single strand conformation polymorphism analysis, PCR-restriction fragment length polymorphism analysis and DNA sequencing. Mutations were identified in 2 of 24 breast tumor samples. One G→A transition resulting in a silent mutation and a single base deletion resulting in a nonsense mutation were detected in one patient. Another breast cancer sample harbored a T→A transition at codon 159. An association between the codon 109 B allele and breast cancer was observed.

Conclusion: Our study indicates that mutational alterations in the p27 gene are rare in human breast cancer. The codon 109 B allele is associated with high-grade tumors.

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Acknowledgments

This study has been supported by the Istanbul University Research Fund (project numbers B-1064/20062001 and T-811/07032000). The authors have provided no information on conflicts of interest directly relevant to the content of this study.

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Authors and Affiliations

  1. Department of Medical Biology, Cerrahpaşa Medical School, Istanbul University, Istanbul, Turkey

    Hatice Tigli & Nur Buyru

  2. Department of Basic Oncology, Oncology Institute, Istanbul University, 34390, Capa, Istanbul, Turkey

    Nejat Dalay

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  1. Hatice Tigli
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  2. Nur Buyru
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  3. Nejat Dalay
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Correspondence to Nejat Dalay.

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Tigli, H., Buyru, N. & Dalay, N. Molecular Analysis of the P27/Kip1 Gene in Breast Cancer. CNS Drugs 9, 17–21 (2005). https://doi.org/10.2165/00066982-200509010-00003

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