Summary
The plasmid-mediated β-lactamase in Branhamella catarrhalis (BRO-1), also occurring in Moraxella nonliquefaciens, differs from other known plasmid-mediated β-lactamases in Gram-negative bacteria regarding substrate profile and isoelectric point.
B. catarrhalis strains previously reported to produce β-lactamases deviating from BRO-1 were tested, and the β-lactamases did not differ significantly from BRO-1 in substrate profile, isoelectric point or relative substrate affinity index (RSAI). Further investigations of strains of various geographic origin should be undertaken. RSAI seems to be a useful tool for screening of β-lactamases in B. catarrhalis since values for a large number of strains can easily be determined.
The previously reported conjugational transfer of BRO-1 production within species B. catarrhalis and from M. nonliquefaciens to B. catarrhalis was confirmed. Four bands of extrachromosomal DNA were regularly detected by agarose gel electrophoresis in β-lactamase-producing as well as in β-lactamase-negative strains of B. catarrhalis and M. non-liquefaciens, provided that the excessive nuclease activity in the preparations was inhibited.
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References
Ambler RP, Scott GK. Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K. Proceedings of the National Academy of Sciences, USA 75: 3732–3736, 1978
Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Research 7: 1513–1523, 1979
Buu Hoi-Dang Van A, Brive-Le Bouguenec C, Barthelemy M, Labia R. Novel beta-lactamase from Branhamella catarrhalis. Annales de Microbiologie 129B: 397–406, 1978
Eliasson I, Kamme C. Characterization of the plasmid-mediated β-lactamase in Branhamella catarrhalis, with special reference to substrate affinity. Journal of Antimicrobial Chemotherapy 15: 139–149, 1985
Eliasson I, Kamme C, Prellner K. Prevalence and transfer of β-lactamase production in the upper respiratory tract flora. A study of fifty children undergoing adenoidectomy. In press, 1986
Elwell LP, De Graaff J, Seibert D, Falkow S. Plasmid-linked ampicillin resistance in Haemophilus influenzae type b. Infection and Immunity 12: 404–410, 1975
van Embden JDA, van Klingeren B, Dessens-Kroon M, van Wijngaarden LJ. Penicillinase-producing Neisseria gonorrhoeae in the Netherlands: Epidemiology and genetic and molecular characterization of their plasmids. Antimicrobial Agents and Chemotherapy 18: 789–797, 1980
Farmer T, Reading C. Beta-lactamases of Branhamella catarrhalis and their inhibition by clavulanic acid. Antimicrobial Agents and Chemotherapy 21: 506–508, 1982
Kamme C, Vang M, Ståhl S. Transfer of beta-lactamase production in Branhamella catarrhalis. Scandinavian Journal of Infectious Diseases 15: 225–226, 1983
Kamme C, Vang M, Ståhl S. Intrageneric and intergeneric transfer of Branhamella catarrhalis β-lactamase production. Scandinavian Journal of Infectious Diseases 16: 153–155, 1984
Labia R, Guionie M, Barthélémy M, Philippon A. Properties of three carbenicillin-hydrolysing β-lactamases (CARB) from Pseudomonas aeruginosa: identification of a new enzyme. Journal of Antimicrobial Chemotherapy 7: 49–56, 1981
Levesque R, Roy P, Letarte R, Pechere J-C. A plasmid-mediated cephalosporinase from Achromobacter species. Journal of Infectious Diseases 145: 753–761, 1982
Matthew M, Hedges RW, Smith JT. Types of beta-lactamase determined by plasmid in gram-negative bacteria. Journal of Bacteriology 138: 657–662, 1979
Medeiros AA. β-Lactamases. British Medical Bulletin 40: 18–27, 1984
Medeiros AA, Cohenfors M, Jacoby GA. Five novel plasmid-de-termined β-lactamases. Antimicrobial Agents and Chemotherapy 27: 715–719, 1985
Meyers JA, Sanchez D, Elwell LP, Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology 127: 1529–1537, 1976
Percival A, Corkill JE, Rowlands J, Sykes RB. Pathogenicity of and beta-lactamase production by Branhamella (Neisseria) catarrhalis. Lancet 2: 1175, 1977
Pintado C, Salvador C, Rotger R, Nombela C. Multiresistance plasmid from commensal Neisseria strains. Antimicrobial Agents and Chemotherapy 1: 120–124, 1985
Piot P, Roberts M. Beta-lactamase production in commensal Neisseriaceae. Lancet 1: 619, 1979
Rubin LG, Medeiros AA, Yolken RH, Moxon ER. Ampicillin treatment failure of apparently β-lactamase-negative Haemophilus influenzae type b meningitis due to novel β-lactamase. Lancet 2: 1008–1010, 1981
Simpson IN, Plested SJ. The origin and properties of beta-lactamase satellite bands seen in isoelectric focusing. Journal of Antimicrobial Chemotherapy 12: 127–131, 1983
Simpson IN, Plested SJ, Budin-Jones MJ, Lees J, Hedges RW, et al. Characterization of a novel plasm id-mediated β-lactamase and its contribution to β-lactam resistance in Pseudomonas aeruginosa. FEMS Microbiology Letters 19: 23–27, 1983
Stobberingh EE, Davies BI, van Boven CPA. Branhamella catarrhalis: antibiotic sensitivities and β-lactamases. Journal of Antimicrobial Chemotherapy 13: 55–64, 1984
Takahashi I, Tsukamoto K, Harada M, Sawai T. Carbenicillinhydrolysing penicillinases of Proteus mirabilis and the PSE-type penicillinases of Pseudomonas aeruginosa. Microbiology and Immunology 27: 995–1004, 1983
Thorne G, Farrar Jr EF. Transfer of ampicillin resistance between strains of Haemophilus influenzae type B. Journal of Infectious Diseases 132: 276–281, 1975
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Kamme, C., Eliasson, I., Knutson, B.K. et al. Plasmid-Mediated β-Lactamase in Branhamella catarrhalis . Drugs 31 (Suppl 3), 55–63 (1986). https://doi.org/10.2165/00003495-198600313-00013
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DOI: https://doi.org/10.2165/00003495-198600313-00013