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Problems and Pitfalls in the Assay of Pentamidine

Implications for Clinical Use

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Summary

Of the existing techniques, HPLC analysis of pentamidine allows for the most sensitive and rapid screening of clinical samples. However, it is important to test several extraction and chromatographic procedures to determine which system works consistently well for the particular samples being routinely analysed. Importantly, pentamidine is metabolised in rats and may be metabolised in humans, so an HPLC assay should separate pentamidine and its metabolites adequately. Capillary zone electrophoresis shows promise as a technique that may surpass HPLC for analysing pentamidine, but remains, at this point, unsuitable for routine work due to insufficient validation.

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Authors and Affiliations

  1. Departments of Parasitology, Epidemiology and Pathology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

    Bradley J. Berger, Leonard Henry, James Edwin Hall &  Richard R. Tidwell (Professor of Pathology and Medicinal Chemistry, Director)

  2. Dionex Corporation, Smyrna, Georgia, USA

    Bradley J. Berger, Leonard Henry, James Edwin Hall &  Richard R. Tidwell (Professor of Pathology and Medicinal Chemistry, Director)

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  1. Bradley J. Berger
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  2. Leonard Henry
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  3. James Edwin Hall
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  4. Richard R. Tidwell
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Berger, B.J., Henry, L., Hall, J.E. et al. Problems and Pitfalls in the Assay of Pentamidine. Clin. Pharmacokinet. 22, 163–168 (1992). https://doi.org/10.2165/00003088-199222030-00001

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  • DOI: https://doi.org/10.2165/00003088-199222030-00001

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