Sports Medicine

, Volume 11, Issue 1, pp 6–19 | Cite as

Muscle Glycogen Synthesis Before and After Exercise

  • John L. Ivy
Review Article

Summary

The importance of carbohydrates as a fuel source during endurance exercise has been known for 60 years. With the advent of the muscle biopsy needle in the 1960s, it was determined that the major source of carbohydrate during exercise was the muscle glycogen stores. It was demonstrated that the capacity to exercise at intensities between 65 to 75% V̇O2max was related to the pre-exercise level of muscle glycogen, i.e. the greater the muscle glycogen stores, the longer the exercise time to exhaustion. Because of the paramount importance of muscle glycogen during prolonged, intense exercise, a considerable amount of research has been conducted in an attempt to design the best regimen to elevate the muscle’s glycogen stores prior to competition and to determine the most effective means of rapidly replenishing the muscle glycogen stores after exercise. The rate-limiting step in glycogen synthesis is the transfer of glucose from uridine diphosphate-glucose to an amylose chain. This reaction is catalysed by the enzyme glycogen synthase which can exist in a glucose-6-phosphate-dependent, inactive form (D-form) and a glucose-6-phosphate-independent, active form (I-form). The conversion of glycogen synthase from one form to the other is controlled by phosphorylation-dephosphorylation reactions.

The muscle glycogen concentration can vary greatly depending on training status, exercise routines and diet. The pattern of muscle glycogen resynthesis following exercise-induced depletion is biphasic. Following the cessation of exercise and with adequate carbohydrate consumption, muscle glycogen is rapidly resynthesised to near pre-exercise levels within 24 hours. Muscle glycogen then increases very gradually to above-normal levels over the next few days. Contributing to the rapid phase of glycogen resynthesis is an increase in the percentage of glycogen synthase I, an increase in the muscle cell membrane permeability to glucose, and an increase in the muscle’s sensitivity to insulin. The slow phase of glycogen synthesis appears to be under the control of an intermediate form of glycogen synthase that is highly sensitive to glucose-6-phosphate activation. Conversion of the enzyme to this intermediate form may be due to the muscle tissue being constantly exposed to an elevated plasma insulin concentration subsequent to several days of high carbohydrate consumption.

For optimal training performance, muscle glycogen stores must be replenished on a daily basis. For the average endurance athlete, a daily carbohydrate consumption of 500 to 600g is required. This results in a maximum glycogen storage of 80 to 100 µmol/g wet weight. To glycogen supercompensate in preparation for competition, the muscle glycogen stores must first be exercise-depleted. This should then be followed with a natural training taper. During the first 3 days of tapering, a mixed diet composed of 40 to 50% carbohydate should be consumed. During the last 3 days of tapering, a diet consisting of 70 to 80% carbohydrate is consumed. This procedure results in muscle glycogen concentrations that are comparable to those produced by more rigorous regimens that can result in chronic fatigue and injury. For rapid resynthesis of muscle glycogen stores, a carbohydrate supplement in excess of 1 g/kg bodyweight should be consumed immediately after competition or after a training bout. Continuation of supplementation every 2 hours will maintain a maximal rate of storage up to 6 hours after exercise. Supplements composed of glucose or glucose polymers are more effective for the replenishment of muscle glycogen stores after exercise than supplements composed of predominantly fructose. However, some fructose is recommended because it is more effective than glucose in the replenishment of liver glycogen.

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References

  1. Adolfsson S. Effect of contraction in vitro on glycogen content and glycogen synthetase activity in muscle. Acta Physiologica Scandinavica 88: 189–197, 1973PubMedCrossRefGoogle Scholar
  2. Ahlborg BG, Bergström J, Brohult J, Ekelund LG, Hultman E, et al. Human muscle glycogen content and capacity for prolonged exercise after different diets. Foersvarsmedicin 3: 85–99, 1967aGoogle Scholar
  3. Ahlborg B, Bergström J, Ekelund LG, Hultman E. Muscle glycogen and muscle electrolytes during prolonged physical exercise. Acta Physiologica Scandinavica 70: 129–142, 1967bCrossRefGoogle Scholar
  4. Bergström J, Hermansen L, Hultman E, Saltin B. Diet, muscle glycogen and physical performance. Acta Physiologica Scandinavica 71: 140–150, 1967PubMedCrossRefGoogle Scholar
  5. Bergström J, Hultman E. A study of the glycogen metabolism during exercise in man. Scandinavian Journal of Clinical and Laboratory Investigation 19: 218–226, 1967aPubMedCrossRefGoogle Scholar
  6. Bergström J, Hultman E. Muscle glycogen synthesis after exercise: an enhancing factor localized to the muscle cells in man. Nature 210: 309–310, 1967bCrossRefGoogle Scholar
  7. Bergström J, Hultman E. Synthesis of muscle glycogen in man after glucose and fructose infusion. Acta Medica Scandinavica 182: 93–107, 1967cPubMedCrossRefGoogle Scholar
  8. Bergström J, Hultman E, Roch-Norlund AE. Muscle glycogen synthetase in normal subjects. Scandinavian Journal of Clinical and Laboratory Investigation 29: 231–236, 1972PubMedCrossRefGoogle Scholar
  9. Blom PCS, Høstmark AT, Vaage O, Kardel KR, Maehlum S. Effect of different post-exercise sugar diets on the rate of muscle glycogen synthesis. Medicine and Science in Sports and Exercise 19: 491–496, 1987PubMedGoogle Scholar
  10. Christensen EH, Hansen O. Arbeitsfahigket und Ermundung. Scandinavian Archives of Physiology 81: 160–171, 1939aCrossRefGoogle Scholar
  11. Christensen EH, Hansen O. Hypoglykamie, Arbeitsfahigkeit und Ermundung. Scandinavian Archives of Physiology 81: 172–179, 1939bCrossRefGoogle Scholar
  12. Costill DL, Bowers R, Branam G, Sparks K. Muscle glycogen utilization during prolonged exercise on successive days. Journal of Applied Physiology 31: 834–838, 1971PubMedGoogle Scholar
  13. Costill DL, Sherman WM, Fink WJ, Maresh C, Witten M, et al. The role of dietary carbohydrate in muscle glycogen resynthesis after strenuous running. American Journal of Clinical Nutrition 34: 1831–1836, 1981PubMedGoogle Scholar
  14. Danforth WH. Glycogen synthetase activity in skeletal muscle: interconversion of two forms and control of glycogen synthesis. Journal of Biological Chemistry 240: 588–593, 1965PubMedGoogle Scholar
  15. Fell RD, Terblanche SE, Ivy JL, Young JC, Holloszy JO. Effect of muscle glycogen content on glucose uptake following exercise. Journal of Applied Physiology 52: 434–437, 1982PubMedGoogle Scholar
  16. Fischer EH, Heilmeyer LMG, Haschke RH. Phosphorylase and the control of glycogen degradation. Current Topics in Cellular Regulation 4: 211–251, 1971Google Scholar
  17. Grollman S. A study of oxygen debt in the albino rat. Journal of Experimental Zoology 128: 511–523, 1955CrossRefGoogle Scholar
  18. Guinovart JJ, Salavert A, Massague J, Ciudad CJ, Salsas E, Itarte E. Glycogen synthase: a new activity ratio assay expressing a high sensitivity to the phosphorylation state. FEBS Letters 106: 284–288, 1979PubMedCrossRefGoogle Scholar
  19. Hermansen L, Hultman E, Saltin B. Muscle glycogen during prolonged severe exercise. Acta Physiologica Scandinavica 71: 334–346, 1965Google Scholar
  20. Hodges RE, Krehl WA. The role of carbohydrates in lipid metabolism. American Journal of Clinical Nutrition 17: 334–346, 1965PubMedGoogle Scholar
  21. Holloszy JO, Narahara HT. Studies of tissue permeability. X. Changes in permeability to 3-methylglucose associated with contraction of isolated frog muscle. Journal of Biological Chemistry 240: 3493–3500, 1965PubMedGoogle Scholar
  22. Huang K-P, Huang FL. Phosphorylation of rabbit skeletal muscle glycogen synthase by cyclic AMP-dependent protein kinase and dephosphorylation of the synthase by phosphatases. Journal of Biological Chemistry 255: 3141–3147, 1980PubMedGoogle Scholar
  23. Hultman E. Studies on muscle metabolism of glycogen and active phosphate in man with special reference to exercise and diet. Scandinavian Journal of Clinical and Laboratory Investigation 19(Suppl. 94): 1–63, 1967Google Scholar
  24. Hunt JN, Smith JL, Jiang CL. Effect of meal volume and energy density on the gastric emptying of carbohydrate. Gastroenterology 89: 1326–1330, 1985PubMedGoogle Scholar
  25. Ivy JL. Role of insulin during exercise-induced glycogenesis in muscle: effect on cyclic AMP. American Journal of Physiology 236: E509–E513, 1977Google Scholar
  26. Ivy JL. The insulin-like effect of muscle contraction. In Pandolf KB (Ed.) Exercise and sports science reviews, Vol. 15, pp. 29–51, Macmillan, New York, 1987Google Scholar
  27. Ivy JL, Frishberg BA, Farrell SW, Miller WJ, Sherman WM. Effects of elevated and exercise-reduced muscle glycogen levels on insulin sensitivity. Journal of Applied Physiology 59: 154–159, 1985PubMedGoogle Scholar
  28. Ivy JL, Holloszy JO. Persistent increase in glucose uptake by rat skeletal muscle following exercise. American Journal of Physiology 241: C200–C203, 1981PubMedGoogle Scholar
  29. Ivy JL, Katz AL, Cutler CL, Sherman WM, Coyle EF. Muscle glycogen synthesis after exercise: effect of time of carbohydrate ingestion. Journal of Applied Physiology 64: 1480–1485, 1988aPubMedGoogle Scholar
  30. Ivy JL, Lee MC, Brozinick JT, Reed MJ. Muscle glycogen storage after different amounts of carbohydrate ingestion. Journal of Applied Physiology 65: 2018–2023, 1988bPubMedGoogle Scholar
  31. Ivy JL, Sherman WM, Miller W, Farrall S, Frishberg B. Glycogen synthesis: effect of diet and training. In Knuttgen et al. (Eds) Biochemistry of exercise, pp. 291–296, Human Kinetics, Champaign, IL, 1983Google Scholar
  32. Keizer HA, Kuipers H, van Kranenburg G, Guerten P. Influence of lipid and solid meals on muscle glycogen resynthesis, plasma fuel hormone response, and maximal physical work capacity. International Journal of Sports Medicine 8: 99–104, 1986CrossRefGoogle Scholar
  33. Kochan RG, Lamb DR, Lutz SA, Perrill CV, Reimann EM, Schlender KK. Glycogen synthase activation in human skeletal muscle: effects of diet and exercise. American Journal of Physiology 236: E660–E666, 1979PubMedGoogle Scholar
  34. Kochan RG, Lamb DR, Reimann EM, Schlender KK. Modified assays to detect activation of glycogen synthase following exercise. American Journal of Physiology 240: E197–E202, 1981PubMedGoogle Scholar
  35. Larner J, Rosell-Perez M, Friedman DL, Craig JW. Insulin and the control of UDPG-α-glucan transglucosylase activity. In Whelan & Cameron (Eds) Control of glycogen metabolism, pp. 273–293, Little, Brown, Boston, 1963Google Scholar
  36. Levine SA, Gordon B, Derrick CL. Some changes in the chemical constituents of the blood following a marathon race: with special reference to the development of hypoglycemia. Journal of the American Medical Association 82: 1778–1779, 1924CrossRefGoogle Scholar
  37. Maehlum S, Felig P, Wahren J. Splanchnic glucose and muscle glycogen metabolism after glucose feeding post-exercise recovery. American Journal of Physiology 235: E255–E260, 1978PubMedGoogle Scholar
  38. Maehlum S, Hermansen L. Muscle glycogen during recovery after prolonged severe exercise in fasting subjects. Scandinavian Journal of Clinical and Laboratory Investigation 38: 557–560, 1978PubMedCrossRefGoogle Scholar
  39. Maehlum S, Hestmark AT, Hermansen L. Synthesis of muscle glycogen during recovery after prolonged severe exercise in diabetic and non-diabetic subjects. Scandinavian Journal of Clinical and Laboratory Investigation 37: 309–316, 1977PubMedCrossRefGoogle Scholar
  40. Nesher R, Karl SE, Kipnis DM. Dissociation of effects of insulin and contraction on glucose transport in rat epitrochlearis muscle. American Journal of Physiology 249: C226–C232, 1985PubMedGoogle Scholar
  41. Nilsson LH, Hultman E. Liver and muscle glycogen in man after glucose and fructose infusion. Scandinavian Journal of Clinical and Laboratory Investigation 33: 5–10, 1974PubMedCrossRefGoogle Scholar
  42. Reed MJ, Brozinick JT, Lee MC, Ivy JL. Muscle glycogen storage postexercise: effect of mode of carbohydrate administration. Journal of Applied Physiology 66: 720–726, 1989PubMedGoogle Scholar
  43. Richter EA, Garetto LP, Goodman NM, Ruderman NB. Muscle glycogen metabolism following exercise in the rat: increased sensitivity to insulin. Journal of Clinical Investigation 69: 785–793, 1982PubMedCrossRefGoogle Scholar
  44. Richter EA, Garetto LP, Goodman NM, Ruderman NB. Enhanced muscle glycogen metabolism after exercise: modulation by local factors. American Journal of Physiology 246: E476–E482, 1984PubMedGoogle Scholar
  45. Roach PJ, Lamer J. Rabbit skeletal muscle glycogen synthase. II. Enzyme phosphorylation state and effector concentrations as interacting control parameters. Journal of Biological Chemistry 251: 1920–1925, 1976PubMedGoogle Scholar
  46. Roach PJ, Larner J. Covalent phosphorylation in the regulation of glycogen synthase activity. Molecular and Cellular Biochemistry 15: 179–200, 1977PubMedCrossRefGoogle Scholar
  47. Roch-Norlund AE, Bergström J, Hultman E. Muscle glycogen and glycogen synthetase in normal subjects and in patients with diabetes mellitus: effect of intravenous glucose and insulin administration. Scandinavian Journal of Clinical and Laboratory Investigation 30: 77–84, 1972PubMedCrossRefGoogle Scholar
  48. Sherman WM. Carbohydrates, muscle glycogen and muscle glycogen supercompensation. In Williams MH (Ed.) Ergogenic aids in sports, pp. 3–26, Human Kinetics, Champaign, IL, 1983Google Scholar
  49. Sherman WM, Costill DL, Fink WJ, Miller JM. The effect of exercise and diet manipulation on muscle glycogen and its subsequent utilization during performance. International Journal of Sports Medicine 2: 114–118, 1981PubMedCrossRefGoogle Scholar
  50. Sherman WM, Lamb DR. Nutrition and prolonged exercise. In Lamb DR (Ed.) Perspectives in exercise science and sports medicine: prolonged exercise, pp. 213–277, Benchmark Press, Indianapolis, IN, 1988Google Scholar
  51. Shreeve WW, Baker N, Miller M, et al. 14C studies in carbohydrate metabolism: oxidation of glucose in diabetic human subjects. Metabolism 5: 22–29, 1956PubMedGoogle Scholar
  52. Szanto S, Yudkin J. The effect of dietary sucrose on blood lipids, serum insulin, platelet adhesiveness and body weight in human volunteers. Postgraduate Medical Journal 45: 602–607, 1969PubMedCrossRefGoogle Scholar
  53. Terjung RL, Baldwin KM, Winder WW, Holloszy JO. Glycogen in different types of muscle and in liver after exhausting exercise. American Journal of Physiology 226: 1387–1391, 1974PubMedGoogle Scholar
  54. Yakovlev NN. The effect of regular muscular activity on enzymes of glycogen, and glucose-6-phosphate in muscles and liver. Biochemistry 33: 602–607, 1968Google Scholar
  55. Young AA, Bogardus C, Stone K, Mott DM. Insulin response of components of whole-body and muscle carbohydrate metabolism in humans. American Journal of Physiology 254; E231–E236, 1988PubMedGoogle Scholar
  56. Zakin D, Herfman RH, Gordon WC. The conversion of glucose and fructose to fatty acids in the human liver. Biochemical Medicine 2: 427–437, 1969CrossRefGoogle Scholar

Copyright information

© Adis International Limited 1991

Authors and Affiliations

  • John L. Ivy
    • 1
  1. 1.Exercise Physiology and Metabolism Laboratory, Department of KinesiologyUniversity of Texas at AustinAustinUSA
  2. 2.Department of KinesiologyUniversity of Texas at AustinAustinUSA

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