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Molecular Medicine

, Volume 23, Issue 1, pp 258–271 | Cite as

miR-210 Protects Renal Cell Against Hypoxia-induced Apoptosis by Targeting HIF-1 Alpha

  • Li-Li Liu
  • Dahu Li
  • Yun-Ling He
  • Yan-Zhao Zhou
  • Sheng-Hui Gong
  • Li-Ying Wu
  • Yong-Qi Zhao
  • Xin Huang
  • Tong Zhao
  • Lun Xu
  • Kui-Wu Wu
  • Ming-Gao Li
  • Ling-Ling Zhu
  • Ming Fan
Research Article

Abstract

The kidney is vulnerable to hypoxia-induced injury. One of the mechanisms underlying this phenomenon is cell apoptosis triggered by hypoxia-inducible factor-1- α (HIF-1α) activation. MicroRNA-210 (miR-210) is known to be induced by HIF-1α and can regulate various pathological processes, but its role in hypoxic kidney injury remains unclear. Here, in both rat systemic hypoxia and local kidney hypoxia models, we found miR-210 levels were upregulated significantly in injured kidney, especially in renal tubular cells. A similar increase was observed in hypoxia-treated human renal tubular HK-2 cells. We also verified that miR-210 can directly suppress HIF-1α expression by targeting the 3′ untranslated region of HIF-1α mRNA in HK-2 cells in severe hypoxia. Accordingly, miR-210 overexpression caused significant inhibition of the HIF-1α pathway and attenuated apoptosis caused by hypoxia, while miR-210 knockdown exerted the opposite effect. Taken together, our findings verify that miR-210 is involved in the molecular response in hypoxic kidney lesions in vivo and attenuates hypoxia-induced renal tubular cell apoptosis by targeting HIF-1α directly and suppressing HIF-1α pathway activation in vitro.

Notes

Acknowledgments

This work was supported by the grants from the Natural Science Foundation of China (Nos. 81430044 and 31271205) and the National Basic Research Program of China (Nos. 2011CB910800 and 2012CB518200).

Supplementary material

10020_2017_2301258_MOESM1_ESM.pdf (279 kb)
Supplementary material, approximately 279 KB.

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Authors and Affiliations

  • Li-Li Liu
    • 1
    • 2
  • Dahu Li
    • 1
  • Yun-Ling He
    • 1
  • Yan-Zhao Zhou
    • 1
  • Sheng-Hui Gong
    • 1
  • Li-Ying Wu
    • 1
  • Yong-Qi Zhao
    • 1
  • Xin Huang
    • 1
  • Tong Zhao
    • 1
  • Lun Xu
    • 1
  • Kui-Wu Wu
    • 1
  • Ming-Gao Li
    • 2
  • Ling-Ling Zhu
    • 1
    • 3
  • Ming Fan
    • 1
    • 3
    • 4
    • 5
  1. 1.Department of Cognitive ScienceInstitute of Basic Medical SciencesBeijingChina
  2. 2.Navy Aviation and Diving Medical CenterNavy General Hospital of PLABeijingChina
  3. 3.Co-innovation Center of NeuroregenerationNantong UniversityNantongChina
  4. 4.Beijing Institute for Brain DisordersBeijingChina
  5. 5.Department of Brain Protection and PlasticityInstitute of Basic Medical SciencesBeijingChina

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