Role of Glycine N-Methyltransferase in the Regulation of T-Cell Responses in Experimental Autoimmune Encephalomyelitis
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Glycine N-methyltransferase (GNMT) is known for its function as a tumor suppressor gene. Since 100% of female Gnmt−/− mice developed hepatocellular carcinoma, we hypothesized that Gnmt−/− mice may have defective immune surveillance. In this study, we examined the immune modulation of GNMT in T-cell responses using experimental autoimmune encephalomyelitis (EAE). The results showed that EAE severity was reduced significantly in Gnmt−/− mice. Pathological examination of the spinal cords revealed that Gnmt−/− mice had significantly lower levels of mononuclear cell infiltration and demyelination than the wild-type mice. In addition, quantitative real-time PCR showed that expression levels of proinflammatory cytokines, including interferon (IFN)-γ and interleukin (IL)-17A, were much lower in the spinal cord of Gnmt−/− than in that of wild-type mice. Accordingly, myelin oligodendrocyte glycoprotein (MOG)-specific T-cell proliferation and induction of T-helper (Th)1 and Th17 cells were markedly suppressed in MOG35–55-induced Gnmt−/− mice. Moreover, the number of regulatory T (Treg) cells was increased significantly in these mice. When the T-cell receptor was stimulated, the proliferative capacity and the activation status of mTOR-associated downstream signaling were decreased significantly in Gnmt−/− CD4+ T cells via an IL-2- and CD25-independent manner. Moreover, GNMT deficiency enhanced the differentiation of Treg cells without affecting the differentiation of Th1 and Th17 cells. Furthermore, the severity of EAE in mice adoptive transferred with GNMT-deficient CD4+ T cells was much milder than in those with wild-type CD4+ T cells. In summary, our findings suggest that GNMT is involved in the pathogenesis of EAE and plays a crucial role in the regulation of CD4+ T-cell functions.
Glycine N-methyltransferase (GNMT, EC 18.104.22.168) regulates the ratio of S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH) and serves as a folate-binding protein (1,2). GNMT binds environmental carcinogens and prevents the DNA adduct formation and cytotoxicity induced by these carcinogens (3,4). Recently, we reported that GNMT was involved in the mammalian target of rapamycin (mTOR) signal transduction pathway and in intracellular cholesterol trafficking by interacting with DEP domain-containing mTOR-interacting protein (DEPTOR) and Neimann-Pick type C2 (NPC2) proteins, respectively (5,6). Moreover, expression of GNMT was downregulated in human hepatocellular carcinoma (HCC), and 50% male and 100% female Gnmt knockout (Gnmt−/−) mice spontaneously developed HCC (7, 8, 9). Therefore, GNMT is a tumor suppressor gene for HCC.
Since a high percentage of Gnmt−/− mice developed HCC, we hypothesized that GNMT deficiency may affect the immune surveillance system. Accumulating evidence suggests that GNMT plays a critical role in the regulation of immune responses. Previously, we reported that compared with the wild-type mice, Gnmt−/− mice had significantly lower numbers of white blood cells, neutrophils and monocytes (10). In addition, cell numbers of lymphocytes, basophils and eosinophils were decreased slightly in knockout mice compared with wildtype mice (10). In Gnmt and apolipoprotein E double knockout mice, GNMT deficiency modulated the functions of macrophages and exacerbated the pathogenesis of hyperlipidemia, inflammation and atherosclerosis, indicating that GNMT regulates the inflammatory responses by modulating the function of macrophages (11). Moreover, GNMT deficiency increased the susceptibility to dextran sulfate sodium (DSS)-induced colitis in mice (12). Natural killer (NK) cells from Gnmt−/− mice were activated spontaneously, expressed more tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and had stronger cytotoxic activity, suggesting that they contributed to a proinflammatory environment in the liver (13). These lines of evidence indicate that GNMT is involved in the pathogenesis of inflammatory processes. However, whether GNMT regulates the functions of CD4+ T-helper (Th) cell lineages is still unclear.
Multiple sclerosis (MS) is a chronic inflammatory, demyelinating, T-cell-mediated autoimmune disease of the central nervous system (14). In addition to an immunological etiology, it has been reported that deficiency of SAM, a major methyl group, appears to be a pathogenic mechanism in various neurological disorders, including MS (15). Many of these diseases containing a myelination defect or demyelination were found to be associated with inborn error of metabolism, which affected the methyl transfer pathway (16,17), suggesting that metabolic events also played a role in the pathogenesis of MS. Murine experimental autoimmune encephalomyelitis (EAE), a widely used animal model for human MS, provides a crucial tool for improving our understanding and treatment of multiple sclerosis (18). Th1 and Th17 cells are well documented to promote the progression of autoimmune encephalomyelitis (21). Our study aims to further investigate the modulatory role of GNMT in the regulation of T-cell functions during EAE pathogenesis.
Materials and Methods
C57BL/6 mice were purchased from the National Laboratory Animal Center in Taipei, Taiwan. Gnmt−/− mice in a C57BL/6 background were generated as described previously (10). B6. Rag1−/− mice were originally acquired from The Jackson Laboratory (Bar Harbor, ME, USA). Eight- to 12-wk-old female mice were used for all experiments. All mice were housed in a specific pathogen-free facility in microisolator cages that contained sterilized food, autoclaved bedding and water. All animal protocols were reviewed and approved by the Institutional Animal Care and Use Committee of National Yang-Ming University.
Induction of EAE
To induce EAE, adult female mice (n = 7–12/group) were immunized subcutaneously with 200 µg of myelin oligodendrocyte glycoprotein (MOG35–55) peptide (MEVGWYRSPFSRVVHLYRNGK, >95% purity; Angene Biotech, Taiwan) emulsified in complete Freund’s adjuvant containing 4 mg/mL Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI, USA) in one flank on d 0 and in the other on d 7. Pertussis toxin (PTx; Sigma-Aldrich, St. Louis, MO, USA) (500 ng) was injected intraperitoneally on d 0 and 2 postimmunization. Following the first immunization, the severity of EAE was monitored and scored in a blinded manner on a scale of 0 to 5 according to the following criteria: 0, no clinical signs; 0.5, partially limp tail; 1, paralyzed tail; 2, loss in coordinated movement, hind limb weakness; 2.5, one hind limb paralyzed; 3, both hind limbs paralyzed; 3.5, hind limbs paralyzed, forelimbs weakness; 4, forelimbs paralyzed; 5, moribund and death (22).
Pathologic and Immunohistochemical (IHC) Examinations
On d 17 postimmunization, mice (n = 4/group) were perfused with phosphate-buffered saline (PBS) transcardially. Then, their spinal cords were harvested and fixed in 4% formalin. The fixed tissues were embedded in paraffin wax. Five-micrometer sections were stained with hematoxylin and eosin (H&E), CD45 or Luxol fast blue (LFB) for the analyses of inflammation and demyelination, respectively. Detailed procedures for IHC staining have been described previously (4). Rat anti-mouse CD45 (30-F11) antibody (BD Pharmingen [BD Biosciences, San Jose, CA, USA]) was used for staining. Slides were assessed in a blind fashion for inflammation and demyelination by the pathologist as follows (23). For inflammation: 0, none; 1, a few inflammatory cells; 2, organization of perivascular infiltrates; and 3, increasing severity of perivascular cuffing with extension into the adjacent tissue. For demyelination: 0, none; 1, rare foci; 2, a few areas of demyelination; and 3, large (confluent) areas of demyelination.
Gene Expression Analysis by Using Real-time PCR
On d 17 postimmunization, the mice (n = 4/group) were perfused with PBS transcardially before their spinal cords were removed and treated with TRIzol reagent (Invitrogen [Thermo Fisher Scientific, Waltham, MA, USA]). Tissues were homogenized with a tissue lyser (Qiagen, Valencia, CA, USA). The RNA was extracted with TRIzol reagent and was reverse transcribed into cDNA using a Super Script II Reverse Transcriptase Kit (Invitrogen [Thermo Fisher Scientific]). The PCR was performed on a thermal cycler system (Roche Diagnostics, Mannhein, Germany) using the LightCycler First Start DNA Master SYBR Green I system (Roche). The mRNA level was normalized using the β-actin mRNA level as the standard and expressed as fold changes compared with the WT mice.
MOG-Specific T-Cell Proliferation
The suspensions of mononuclear cells from pooled draining lymph nodes (LNs) were prepared on d 9 postimmunization (n = 3/group) and cultured in complete RPMI medium (RPMI 1640 containing heat-inacatived 10% fetal bovine serum, penicillin (100 U/mL), streptomycin (100 µg/mL), nonessential amino acids (0.1 mmol/L), 10 mmol/L HEPES, 55 µmol/L β-mercaptoethanol and 1 mmol/L sodium pyruvate) (Gibco [Thermo Fisher Scientific, Waltham, MA, USA]) in the absence or presence of various concentrations of MOG35–55 peptide. For proliferation, [3H]-thymidine (1 µCi/well; PerkinElmer, Shelton, CT, USA) was added at 24 h, and the incorporated radioactivity was measured after 12 h using a PerkinElmer Wallac 1450 Microβ Trilux Scintillation Counter (PerkinElmer). The amount of [3H]-thymidine incorporation for each group was normalized to that of the unstimulated control, producing a ratio, referred to here as the proliferation index.
Intracellular Cytokine Staining
Mice were euthanized at d 9 (n = 3/group), d 11 (n = 3/group) and d 18 (n = 3/group) postimmunization. Single cell suspensions were prepared from draining LNs of mice. Cells were stimulated with PMA (20 ng/mL, Sigma) and ionomycin (1 µg/mL, Sigma) in the presence of monensin (4 µmol/L, Sigma) for 4 h. One million cells per reaction were stained with fluorochrome-conjugated antibodies specific for murine CD4 (RM4-5) (eBioscience, San Diego, CA, USA). Intracellular cellular cytokine staining was performed as described previously (24). Cells were stained with specific antibodies for murine interferon (IFN)-γ (XMG1.2), interleukin (IL)-17A (TC11-18H10.1) and FoxP3 (FJK-16s) (eBioscience). Cells were analyzed using a FACSCalibur flow cytometer (BD Biosciences) and Flow Jo software (Tree Star, Ashland, OR, USA)
Isolation of Murine CD4+ T Cells
Spleen and LNs were removed from 8- to 12-wk-old mice, pooled and homogenized through a filter. RBC were lysed using the red blood cell lysing buffer. CD4+ T cells were isolated by negative selection using Easy Sep Mouse CD4+ T Cell Enrichment Kit (Stem Cell Technologies, Vancouver, BC, Canada) or using CD4+ T Cell Isolation Kit II (Miltenyi Biotec, Bergisch Gladbach, Germany).
Enzyme-Linked Immunosorbent Assay (ELISA)
The CD4+ T cells were purified as described above and stimulated with anti-CD3/CD28 (1 µg/mL) for 48 h. The supernatants were collected after stimulation and different quantitative ELISAs were performed using paired monoclonal antibodies (mAbs) as recommended by the manufacturer (R&D Systems, Minneapolis, MN, USA).
Cell Stimulation and Western Blot Analysis
The CD4+ T cells were purified from 8- to 12-wk-old mice and stimulated with anti-CD3ε (145-2C11, eBioscience) and anti-CD28 (37.51, eBioscience) Abs. After stimulation, cells were immediately harvested with radioimmunoprecipitation assay (RIPA) lysis buffer containing protease and phosphatase inhibitors. Cell lysates were analyzed by using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membrane according to the protocol reported previously (8). Densitometry was performed using Dolphin View Band Tool (Wealtec Corp., Sparks, NV, USA). The membranes were reactive to the following Abs: phospho-T389 S6K1 (9205), phospho-S2448 mTOR (2971), phospho-T37/T46 4E-BP1 (9459) (Cell Signaling Technology, Beverly, MA, USA), GRAIL (Imgenex [Novus Biologicals LLC, Littleton, CO, USA]) and β-actin (Sigma).
Analysis of the T-Cell Differentiation
The CD4+CD25− T cells were purified from pooled spleen and draining LNs of 8- to 12-wk-old mice (n = 5–9/group) and stimulated with anti-CD3ε (1 µg/mL) and anti-CD28 (1 µg/mL) mAbs under Th1 (IL-12, 10 ng/mL; anti-IL-4 (11B11), 10 µg/mL), Th17 (IL-6, 10 ng/mL; TGF-β, 1 ng/mL; anti-IL-4, 10 µg/mL; anti-IFN-γ (XMG1.2), 10 µg/mL) or induced regulatory T-cell (iTreg) condition (TGF-β, 5 ng/mL; IL-2, 10 ng/mL; anti-IL-4, 10 µg/mL; anti-IFN-γ, 10 µg/mL) for 3 d. The cytokines were purchased from eBioscience. The neutralizing antibodies were purchased from BioLegend (San Diego, CA, USA). Intracellular cytokine staining was performed as described above.
Adoptive Transfer EAE
Female 8- to 12-wk-old mice (n = 5/group) were EAE induced as described above. Draining LNs and spleen cells were harvested 9 d later and cultured in complete RPMI medium containing MOG35–55 (50 µg/mL) for 4 d. After harvesting, CD4+ T cells were purified by negative selection using Easy Sep Mouse CD4+ T Cell Enrichment Kit (Stem Cell Technologies). Viable CD4+ T cells (6 × 106; purity >95%) were then transferred intraperitoneally into female C57BL/6 mice (n = 3–4/group). Mice were given 500 ng of PTx intraperitoneally on d 0 and 2 after transfer.
For adoptive transfer to Rag1−/− recipients, the CD4+ T cells were isolated from 8- to 12-wk-old wild-type and Gnmt−/− mice (n = 5/group) as described above. CD4+ T cells (7 × 106) were injected in the tail vein of adult female Rag1−/− mice (n = 3–4/group). After 24 h, Rag1−/− recipients were induced EAE as described above.
The χ2 test was used for comparison of incidence of EAE. The unpaired two-tailed Student t test was applied for statistical analysis of other experiments in this study. The differences were considered to be significant when p < 0.05. *p < 0.05, **p < 0.01.
All supplementary materials are available online at https://doi.org/www.molmed.org .
GNMT Is Dispensable for T-Cell Activation and Homeostasis in a Steady-State Manner
GNMT was reported to play a critical role in innate immunity (11,13). However, the role of GNMT in adaptive immunity remains unknown. To determine whether GNMT deficiency modulated the T-cell development, we analyzed the percentages of lymphocyte populations (CD4+, CD8+, and CD19+) in the spleen of Gnmt−/− mice. Total numbers of cells in spleen were comparable between Gnmt−/− and wild-type mice (data not shown). Gnmt−/− mice had a slightly higher percentage of CD4+ cells but a slightly lower percentage of CD19+ cells than wild-type mice, respectively (Supplementary Figures 1A, B). Nevertheless, the percentages of CD8+ cells in the spleen were similar between the Gnmt−/− and wild-type mice (see Supplementary Figures 1A, B). Moreover, the percentages of CD4+FoxP3+ regulatory T (Treg) cells were equal between wild-type and Gnmt−/− mice (Supplementary Figures 1C, D). These results suggested that GNMT deletion had a mild impact on lymphocytic populations of spleen. Furthermore, to examine whether GNMT ablation influenced T-cell activation status, we compared the activation marker CD69 in either CD4+ or CD8+ T cells. Our results indicated that the percentages of CD69+CD4+ or CD69+CD8+ were similar between Gnmt−/− and wild-type mice, excluding the role of GNMT in T-cell activation (Supplementary Figures 1E, F). To further investigate whether GNMT deficiency affected T-cell homeostasis, we analyzed effector (CD44hiCD62lo) and memory (CD44hiCD62Lhi) populations in either CD4+ or CD8+ T cells in the spleen. Our results also revealed that wild-type and Gnmt−/− mice had equal frequencies of effector and memory populations in CD4+ or CD8+ T cells of the spleen (Supplementary Figures 1G, H). These results suggested that GNMT deficiency had a dispensable effect in the activation and homeostasis of steady-state T cells in peripheral lymphoid organs.
GNMT Ablation Ameliorates the Disease Severity of EAE
Clinical features of MOG35–55 peptide-induced EAE in WT and GNMT KO mice.
Mean day of onset (Mean ± SEM)
Mean maximal clinical score (Mean ± SEM)
Mean duration of paralysis (score ≥ 2.5) (Mean ± SEM)
10.92 ± 0.7
15.67 ± 1.81
14.3 ± 1.46a
2.17 ± 0.39b
6.42 ± 2.6b
GNMT Deficiency Reduces Inflammation and Demyelination of the CNS
To confirm whether GNMT deletion decreased inflammation and demyelination in inflamed CNS, we used immunohistochemical analysis to further compare leukocyte infiltration and demyelination of inflamed CNS between MOG-immunized Gnmt−/− and wild-type mice. Pathological examination of the spinal cords harvested from 17 d immunized wild-type (average score, 3.23 ± 0.12) and Gnmt−/− mice (average score, 1 ± 0.7) revealed significant differences between these two groups. Degeneration of white matter characterized by vacuolar formation along with extensive infiltration of inflammatory mononuclear cells was seen consistently in spinal cords of wildtype mice. In contrast, degeneration and inflammation was very mild in Gnmt−/− mice (Figure 2A–F, S). Consistently, IHC staining of the common leukocyte marker CD45 showed abundant inflammatory infiltrates in the spinal cords of the wild-type mice, but few or no leukocytes were detected in the Gnmt−/− mice (Figures 2G–L, S).
To assess the degree of demyelination, we examined the integrity of myelin sheaths by Luxol fast blue staining. As shown in Figures 2M–R and S, the MOG-immunized wild-type mice showed multiple areas of pallor, corresponding to the areas with vacuolar degeneration, in the white matter of the spinal cords. In contrast, the white matter of the spinal cords of Gnmt−/− mice was evenly stained throughout, indicating very mild or no demyelination. The pathological findings of the spinal cord of the wild-type and Gnmt−/− mice correlated very well with the clinical observations.
GNMT Deficiency Downregulates Proinflammatory Cytokines in Inflamed CNS
Induction of MOG-Specific T cells Is Suppressed in Gnmt−/− Mice
Th1 and Th17 are critical players in the pathogenesis of autoimmune encephalomyelitis (25). To further investigate the modulatory effect of GNMT deficiency on Th1 and Th17 cells in mice with EAE induction, we analyzed the percentages of Th1 and Th17 cells in MOG35–55-immunized Gnmt−/− mice (d 9 postimmunization). In line with the results of IFN-γ and IL-17A mRNA levels in inflamed CNS, both the percentages of Th1 cells and Th17 cells were reduced significantly in MOG-immunized Gnmt−/− mice when compared with those in wild-type mice (Figures 4B, C). Similarly, decreases in proliferative responses and cytokine production of IFN-γ and IL-17A also were observed in Gnmt−/− mice compared with wild-type mice at d 23 after EAE induction (Supplementary Figures 4A–C). Furthermore, these results suggested that GNMT deficiency suppressed proliferative responses of MOG-specific T cells and induction of Th1 and Th17 cells, further supporting a protective role of GNMT deficiency in EAE.
Loss-of-Function of GNMT Attenuates TCR-Stimulated T-Cell Proliferation via the mTOR Signaling Pathway
It is well demonstrated that TCR/CD3 engagement and CD28 costimulation increase the expression level of IL-2Rα (CD25) and the amount of secreted IL-2 in T cells (27, 28, 29). We next determined whether GNMT deficiency affected the amount of IL-2 production in the supernatant of cultured CD4+ T cells and the mRNA levels of IL-2 and CD25 in those T cells. Our results indicated that the amount of IL-2 production was increased equally in the culture supernatant of GNMT-deficient CD4+ T cells compared with that of wild-type T cells in response to the stimulation of anti-CD3/CD28 (Figure 5B). Moreover, we also found that both wild-type and GNMT-deficient CD4+ T cells had similarly increased expression of IL-2 and CD25 in response to anti-CD3/CD28 stimulation (Figures 5C, D). Therefore, these results suggested that the deficiency of GNMT strikingly decreased CD4+ T-cell proliferation in an IL-2- and CD25-independent manner.
Since Th1 and Th17 cells played important roles in the development and pathogenic processes of EAE (19,20), we investigated whether GNMT deficiency influenced cytokine production of Th1 (IFN-γ) and Th17 (IL-17A) cells in anti-CD3/CD28-stimulated CD4+ T cells. Our results revealed that production of both IFN-γ and IL-17A was reduced significantly in the supernatant of cultured GNMT-deficient CD4+ T cells compared with wild-type T cells (Figures 5E, F). Collectively, these results suggested that GNMT deficiency impaired TCR stimulation-induced T-cell proliferation and dampened Th1-specific IFN-γ and Th17-specific IL-17A production.
Previous studies have shown that mTOR controls nalve CD4+ T-cell proliferation by regulating the protein expression of gene related to anergy in lymphocyte (GRAIL) (30). Previously we found that GNMT regulated the mTOR signaling pathway by interacting with an mTOR inhibitory protein-DEPTOR in HCC cells (5). To investigate whether GNMT deficiency affected the proliferative capacity of CD4+ T cells through the mTOR signaling pathway, we first examined TCR-mediated phosphorylation status of mTOR and its downstream effectors, S6 Kinase 1 (S6K1) and eIF-4E-binding protein 1 (4E-BP1) (31,32) in activated CD4+ T cells. Our results indicated that the TCR stimulation-induced phosphorylation status of mTOR kinase at Ser2448 was equally increased in both wild-type and Gnmt−/− CD4+ T cells (Figure 5G), suggesting a dispensable role of GNMT in this phosphorylation site of mTOR kinase. In contrast, the phosphorylation status of S6K1 at Thr389 and 4E-BP1 at Thr37/46 were suppressed remarkably in GNMT-deficient CD4+ T cells compared with wild-type CD4+ T cells (see Figure 5G), indicating that GNMT deficiency critically modulated TCR stimulation-based phosphorylation status of mTOR downstream signaling, including S6K1 and 4E-BP1. Since activation of the mTOR signaling pathway promoted the proliferation of nalve CD4+ T cell by down-regulating GRAIL expression (30), we examined whether GNMT deficiency regulated the level of GRAIL expression in activated CD4+ T cells. Our results indicated that, in the presence of anti-CD3/CD28 stimulation, GRAIL expression was reduced significantly in activated CD4+ T cells compared with steady-state T cells of wild-type mice (Figures 5G, H). Nevertheless, the expression level of GRAIL was only slightly decreased in activated CD4+ T cells compared with steady-state T cells from Gnmt−/− mice (see Figures 5G, H), which is consistent with an attenuation of T-cell proliferation in GNMT-deficient mice. Collectively, these data suggested that GNMT deficiency attenuated TCR stimulation-activated CD4+ T-cell proliferation at least partly via the mTOR signaling pathway.
GNMT Deficiency Enhances the Differentiation of Treg Cells In Vitro
GNMT Deficiency Increases the Amount of Treg Cells during EAE Development
GNMT Ablation Ameliorates Adoptive Transfer of EAE
We also purified CD4+ T cells from wild-type and Gnmt−/− mice (purity >95%) and transferred them into Rag1-deficient recipient mice, which were subsequently immunized with MOG35–55/CFA one day later. Our results showed that the recipient mice receiving Gnmt−/− CD4+ T cells exhibited significant attenuation of EAE development, characterized by a slightly delayed onset of disease (KO, 12.3 ± 1.15 d; WT, 11 d) and a decreased mean maximal clinical score (KO, 0.5 ± 0.2; WT, 2.83 ± 1.09) compared with mice receiving wild-type CD4+ T cells (Figures 8C, D). Collectively, these results suggested that amelioration of EAE in Gnmt−/− mice was associated, at least in part, with down-regulation of CD4+ T-cell function.
GNMT was first discovered in liver and plays an important role in the regulation of SAM/SAH ratio, liver detoxification pathway and signaling transduction (34). Previously, we generated and characterized a Gnmt−/− mouse model (9,10). Since the Gnmt−/− mice developed HCC spontaneously, we proposed that GNMT is a HCC tumor suppressor gene (9). During the past decade, most functional studies of GNMT focused on the role of GNMT in the detoxification pathways, nutrition and tumorigenesis of HCC (34,35). However, the role of GNMT in regulation of T-cell functions has never been defined. In this study, we showed that deficiency of GNMT significantly ameliorated the severity of EAE and decreased the responses of both MOG-specific Th1 and Th17 cells in the peripheral and central nervous system. In line with these findings, TCR stimulation-induced responses were attenuated in GNMT-deficient CD4+ T cells. Moreover, GNMT ablation markedly enhanced the differentiation of Treg cells. Collectively, our findings uncovered a novel role of GNMT in the regulation of CD4+ T-cell function during EAE pathogenesis.
A potential role of GNMT in immune modulation has been investigated recently using several experimental models. For instance, atherosclerosis is a chronic inflammatory disease resulting from excessive lipid accumulation and a persistent chronic inflammatory process within the arterial wall (36). Using this model, we previously found that genetic deletion of GNMT in apolipoprotein E-deficient mice exacerbated the dysregulation of cholesterol efflux and inflammation in macrophages, thus leading to accelerated progression of atherosclerosis (11). In addition, Gomez-Santos et al. reported that functional loss of GNMT augmented NK cell-mediated inflammation and exacerbated endotoxin-induced acute liver injury (13). Moreover, absence of GNMT exacerbated the deregulation of the inflammatory response in an experimental ulcerative colitis model (12). Collectively, these evidences suggested that GNMT exerts protective antiinflammatory effects in acute inflammation models and during activation of innate immune responses. In contrast to the role of GNMT in innate immunity, in this study, our data showed that ablation of GNMT ameliorated EAE development and attenuated CD4+ T-cell responses. Since EAE is a CD4+ T-cell-mediated inflammatory demyelinating disease of the CNS (26), GNMT may act as a proinflammatory agent during adaptive immune responses. Interestingly, such opposite roles of GNMT in innate and adaptive immune responses has been found in leptin (37). Nevertheless, how GNMT fine tunes innate and adaptive immunity should be investigated further.
In this study, our results revealed that GNMT deficiency significantly ameliorated the severity of EAE and decreased inflammation and demyelination in the CNS. In addition, MOG-induced Gnmt−/− mice showed reduced MOG-specific T-cell proliferation and decreased induction of Th1 and Th17 cells, but had a higher amount of Treg cells. Moreover, the CD4+ T cells from Gnmt−/− mice showed lower TCR stimulation-induced responses than those from wild-type mice. Furthermore, the severity of adoptive transferred EAE by GNMT-deficient CD4+ T cells was lower than that of wildtype CD4+ T cells. Collectively, our findings suggested that impaired development of EAE in Gnmt−/− mice occurred, at least in part, through modulation of CD4+ T-cell function. However, we failed to completely exclude the possibility that the effects of GNMT deficiency in other cell types could also account for impaired EAE development in Gnmt−/− mice. This still needs to be investigated further.
In addition to the aforementioned mechanism, the effects of GNMT deficiency in one carbon metabolism may also contribute to decreased EAE development. The major players in the transmethylation pathway include methyltransferase-mediated donation of methyl groups by SAM and conversion of SAM to SAH, which can act as a potent feedback inhibitor of methyltransferase (38). This reaction is tightly controlled by S-adenosylhomocysteine hydrolase (SAHase), a highly conserved ubiquitous enzyme that hydrolyzes SAH into adenosine and homocysteine (39). It is reported that SAHase inhibitors not only inhibit SAHase but also result in the accumulation of SAH and SAM (40,41). Interestingly, a range of studies found that inhibition of transmethylation by using SAHase inhibitor curtailed the effector function of CD4+ T cells and EAE development (42,43). Moreover, inhibition of methyl transfer enzymes results in a less compact myelin in oligodendroglial cells, and the methylation status of myelin basic protein (MBP)-arginine is associated with stability of myelin structure (44,45). Intriguingly, our Gnmt−/− mice showed an increased level of hepatic SAM, reduced expression of Ahcy (10) and global DNA hypomethylation (4), suggesting that one carbon metabolism may provide another explanation for impaired EAE in Gnmt−/− mice. Further studies are required to elucidate these mechanisms.
It was reported previously that the engagement of TCR and costimulatory receptor-CD28 increases the expression level of CD25 and the amount of IL-2 secretion and then initiates an IL-2-driven proliferation of CD4+ T cells (46). In this study, we showed that deficiency of GNMT significantly attenuated TCR stimulation-induced CD4+ T-cell proliferation, but had no discernible roles in induction of CD25 and IL-2 production, suggesting that GNMT-deficient CD4+ T cells had potentially equivalent IL-2R signaling compared with wild-type T cells. This phenomenon resembles the findings in mTOR−/− and T-Rheb−/− mouse models. T cells lacking mTOR or Rheb expression did not demonstrate a block in proliferation, but instead proliferated more slowly than their wild-type counterparts (47,48). In addition, a previous study reported that prolonged engagement of TCR and CD28 bypasses the requirement of IL-2 and sustains IL-2-independent T-cell proliferation through mTOR signaling (49). Furthermore, mTOR recently has been shown to promote nalve CD4+ T-cell proliferation through degradation of GRAIL (30). Interestingly, our previous study showed GNMT was able to activate mTORC1 downstream signaling by interrupting the interaction between mTOR and DEPTOR (5). Consistent with previous studies, our results revealed that GNMT deficiency impaired TCR stimulation-induced mTOR signaling and thus maintained high levels of GRAIL expression in stimulated CD4+ T cells. Collectively, our results indicated that GNMT deficiency attenuated TCR-stimulated CD4+ T-cell proliferation by decreasing the mTOR signaling pathway mediated GRAIL degradation in an IL-2-independent manner.
In summary, this study portraits a novel role of GNMT in EAE pathogenesis, TCR stimulation-mediated CD4+ T-cell responses and CD4+ T-cell differentiation. In addition, an underlying molecular mechanism of GNMT in the mTOR signaling pathway to modulate CD4+ T-cell proliferation has been described further. Our novel findings are valuable for the development of GNMT-based therapeutic strategies for the treatment of multiple sclerosis.
Parts of the results have been reported at the 2012 Cold Spring Harbor Asia Conferences-Frontiers of Immunology in Health and Diseases.
This study was supported partially by Kaohsiung Medical University Aim for the Top Universities Grant, KMU-TP103E00 and KMU-TP103E09; Aim for the Top 500 Universities Grant, DT103009; NSYSU-KMU JOINT RESEARCH PROJECT, NSYSUKMU103-I010; Progam to Upgrade the R&D Capabilities of Private Universities Grant, MOST103-2632-B-037-001; and Health and Welfare Surcharge of Tobacco Products, Ministry of Health and Welfare Grant, MOHW103-TD-B-111-05. We thank Chuen-Miin Leu for providing reagents, Marcelo Chen for revising the English writing and the staff from the Center of Infectious Disease and Cancer Research (CICAR) of Kaohsiung Medical University for technical assistance.
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