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Molecular Medicine

, Volume 17, Issue 11–12, pp 1323–1337 | Cite as

Histidine Decarboxylase Is Identified as a Potential Biomarker of Intestinal Mucosal Injury in Patients with Acute Intestinal Obstruction

  • Jian-Jun Yang
  • Yan-Lei Ma
  • Peng Zhang
  • Hong-Qi Chen
  • Zhi-Hua Liu
  • Huan-Long Qin
Research Article

Abstract

Various biomarkers currently used for the diagnosis of intestinal mucosal injury (IMI) in patients with acute intestinal obstruction have low sensitivity and specificity. In the present study, IMI, as indicated by the impaired expression of tight junction proteins, including zonula occludens-1, occludin and claudin-1, and inflammation were determined in colonic tissues of patients with 45 strangulated intestinal obstruction (STR-IO) and the adjacent “normal” colonic tissues of 35 patients with colon cancers by quantitative real-time polymerase chain reaction (QRT-PCR), Western blotting, immunohistochemistry and histological examination, respectively. Then, two-dimensional fluorescent difference gel electrophoresis coupled with linear trap quadrupole mass spectrometry was used to screen for potential biomarkers of IMI in the serum samples of 10 STR-IO, 10 simple intestinal obstruction (SIM-IO) and 10 normal healthy controls. A total of 35 protein spots were differentially expressed among the serum samples, and six of the proteins were identified as potential biomarkers. Among the six proteins, histidine decarboxylase (HDC) and ceruloplasmin (CP) were elevated significantly in patients with STR-IO, compared with patients with SIM-IO and healthy controls. Thus, HDC and CP were further validated by QRT-PCR, Western blotting, immunohistochemistry and enzyme-linked immunosorbent assay, respectively, in colonic tissues, serum and urine samples. Finally, the receiver operating characteristic curves were used to show the area under the curves of HDC, CP and several established biomarkers, followed by the determination of the appropriate cutoff values and their sensitivities and specificities. It was shown that for serum and urine, HDC levels achieved sensitivities and specificities compatible to or even greater than those of established biomarkers for the diagnosis of IMI in patients with acute intestinal obstruction, although further validation in a larger cohort is required.

Notes

Acknowledgments

We are grateful to the staff in the Research Center for Proteome Analysis, Shanghai Institutes for Biological Sciences, for technical assistance and the staff in the Bioinformatics Center, Shanghai Institutes for Biological Sciences, for bioinformatic analysis. This work was supported by grants from the Shanghai Science and Technology Development Fund (05DJ14010), the Major Basic Research Program of Shanghai (07DZ19505) and the National 973 Basic Research Program of China (2008CB517403), Shanghai Rising-Star Program (No.11QA1404800) and the National Natural Science Foundation of China (No.81001069). We also thank Medjaden Bioscience Limited for assisting in the preparation of this manuscript.

Supplementary material

10020_2011_17111323_MOESM1_ESM.pdf (120 kb)
Supplementary material, approximately 120 KB.

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Copyright information

© The Feinstein Institute for Medical Research 2011

Authors and Affiliations

  • Jian-Jun Yang
    • 1
  • Yan-Lei Ma
    • 1
  • Peng Zhang
    • 1
  • Hong-Qi Chen
    • 1
  • Zhi-Hua Liu
    • 1
  • Huan-Long Qin
    • 1
  1. 1.Department of SurgeryThe Sixth People’s Hospital Affiliated to Shanghai Jiao Tong UniversityShanghaiP.R. China

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