Molecular Medicine

, Volume 17, Issue 5–6, pp 533–541 | Cite as

Mammal Cells Double Their Total RNAs against Diabetes, Ischemia Reperfusion and Malaria-Induced Oxidative Stress

  • Zhong-Wei Zhang
  • Jian Cheng
  • Fei Xu
  • Ming Yuan
  • Jun-Bo Du
  • Jing Shang
  • Yong Wang
  • Lei Du
  • Zi-Lin Li
  • Shu Yuan
Research Article


Total cellular RNA level is stable usually, although it may increase gradually during growth or decrease gradually under certain stressors. However, we found that mammal cell RNAs could be doubled within 24 h in response to free heme accumulation (ischemia reperfusion and malaria infection) or a high level of glucose treatment (diabetes). Clinical investigations in rats showed that pretreatment with heme (24 h for doubling total RNAs) alleviated oxidative damages caused by diabetes, and pretreatment with glucose (24 h for trebling total RNAs) alleviated oxidative damages caused by ischemia reperfusion or malaria infection. Therefore, this rapid RNA amplification may play an important role in mammal adaptation to diabetes, ischemia reperfusion and malaria infection-derived oxidative stress. This rapid RNA amplification is derived from glucose and heme, but not from their accompanying reactive oxygen species. Hexokinases endure glucose-derived reactive oxygen species accumulation but are not related glucose-derived RNA amplification. In contrast, the TATA box-binding protein (TBP) mediates all glucose- and heme-induced RNA amplification in mammal cells.



We thank Dr. Xiao-Chao Xu (Sichuan University, China) for technical assistance with RNAi to HEK293 cells. This work was supported by the National Key Basic Research “973” Program of China (2009CB118500), National Nature Science Foundation of China (30970214 and 30800071).

Supplementary material

10020_2011_1705533_MOESM1_ESM.pdf (1.4 mb)
Mammal Cells Double Their Total RNAs against Diabetes, Ischemia Reperfusion and Malaria-Induced Oxidative Stress


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Copyright information

© The Feinstein Institute for Medical Research 2011

Authors and Affiliations

  • Zhong-Wei Zhang
    • 1
    • 2
    • 3
  • Jian Cheng
    • 2
    • 3
  • Fei Xu
    • 2
  • Ming Yuan
    • 1
    • 2
    • 4
  • Jun-Bo Du
    • 2
  • Jing Shang
    • 2
  • Yong Wang
    • 3
  • Lei Du
    • 5
  • Zi-Lin Li
    • 6
  • Shu Yuan
    • 1
    • 2
  1. 1.College of Resources and Environmental SciencesSichuan Agriculture UniversityChengduChina
  2. 2.Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life ScienceSichuan UniversityChengduChina
  3. 3.Provincial Key Laboratory for Tibetan Animal Genetic Resources Conservation and Utilization, College of Life Science and TechnologySouthwest University for NationalitiesChengduChina
  4. 4.College of Biology and ScienceSichuan Agriculture UniversityYa’anChina
  5. 5.College of Veterinary MedicineSichuan Agriculture UniversityYa’anChina
  6. 6.Center of Cardiovascular Surgery, Xijing HospitalFourth Military Medical UniversityXi’anChina

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