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Molecular Medicine

, Volume 14, Issue 1–2, pp 55–63 | Cite as

Altered Interleukin-1 Receptor Antagonist and Interleukin-18 mRNA Expression in Myocardial Tissues of Patients with Dilatated Cardiomyopathy

  • Elena Westphal
  • Susanne Rohrbach
  • Michael Buerke
  • Hagen Behr
  • Dorothea Darmer
  • Rolf-Edgar Silber
  • Karl Werdan
  • Harald Loppnow
Research Article

Abstract

Interleukin-1 (IL-1) is a potent regulator of cell proliferation, inflammation, and contraction of cardiovascular cells. It has been proposed that the IL-1/IL-1ra (IL-1 receptor antagonist) ratio influences these functions. Other members of the IL-1 family and the related caspase-1 also contribute to regulation of IL-1-mediated functions. We determined the mRNA expression of caspase-1, caspase-3, IL-1α, IL-1 β, IL-18, IL-1 receptor type I (IL-1-RI), and IL-1ra in left ventricle tissue of hearts from patients with ischemic or dilated cardiomyopathy (ICM or DCM) and in control tissues from unused donor transplant hearts in RT-PCR experiments. We show that the expression of caspase-1, caspase-3, IL-1β, and IL-1-RI mRNA was not different between patients and control tissues. Furthermore, we did not find detectable amounts of IL-1α mRNA in any of these adult myocardial tissues. On the other hand, expression of IL-18 RNA was lower in myocardium of both patient groups compared with control hearts. Furthermore, IL-1ra mRNA expression was significantly lower in tissues of DCM patients compared with ICM patients and controls. This was in line with a trend towards lower IL-1ra protein levels in myocardial tissues of DCM patients. In contrast with the adult tissues discussed above, which did not express IL-1α mRNA, commercially available human fetal tissue expressed IL-1α mRNA. On the other hand IL-1β mRNA was present in fetal and in adult human heart tissue. Our data provide evidence for an altered ratio of IL-1/IL-1ra in DCM patients. This dysregulation may contribute to pathogenesis and/or progression of heart disease by modulating the otherwise balanced IL-1-mediated functions in cardiovascular cells.

Notes

Acknowledgments

We thank Claudia Pilowski and Beate Heinze for their expert technical assistance. This work was supported by a grant (project 06; Forschungsverbund— Myocard Hypertrophie) of the BMBF to K.W. and H.L. and grant Lo385/4-1 of the DFG to H.L.

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Copyright information

© Feinstein Institute for Medical Research 2008

Authors and Affiliations

  • Elena Westphal
    • 1
  • Susanne Rohrbach
    • 2
  • Michael Buerke
    • 1
  • Hagen Behr
    • 1
  • Dorothea Darmer
    • 2
  • Rolf-Edgar Silber
    • 3
  • Karl Werdan
    • 1
  • Harald Loppnow
    • 1
  1. 1.Universitätsklinik und Poliklinik für Innere Medizin III, Forschungslabor (FG6 E01)Martin-Luther-Universität Halle-WittenbergHalle (Saale)Germany
  2. 2.Institut für PathophysiologieMartin-Luther-Universität Halle-WittenbergHalle (Saale)Germany
  3. 3.Universitätsklinik und Poliklinik für Herzund ThoraxchirurgieMartin-Luther-Universität Halle-WittenbergHalle (Saale)Germany

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