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Molecular Medicine

, Volume 13, Issue 7–8, pp 362–370 | Cite as

Differential Gene Expression after Zinc Supplementation and Deprivation in Human Leukocyte Subsets

  • Hajo Haase
  • Dawn J. Mazzatti
  • Andrew White
  • Klaus H. Ibs
  • Gabriela Engelhardt
  • Silke Hebel
  • Jonathan R. Powell
  • Lothar Rink
Proceedings

Abstract

An individual’s zinc status has a significant impact on the immune system, and zinc deficiency, as well as supplementation, modulates immune function. To investigate the effects of zinc on different leukocyte subsets, we used microarray technology to analyze and compare the changes in mRNA expression in cell culture models of monocytes (THP-1), T cells (Jurkat), and B cells (Raji), in response to supplementation for 40 h with 50 µM zinc or 2.5 µM of the membrane-permeant zinc chelator TPEN [N,N,N′,N′- tetrakis-(2-pyridyl-methyl)ethylenediamine], respectively. In each cell type, several hundred genes were identified to be zinc sensitive, but only a total of seven genes were commonly regulated in all three cell lines. The majority of those genes were involved in zinc homeostasis, and none in immune function. Nevertheless, further analysis revealed that zinc affects entire functional networks of genes that are related to proinflammatory cytokines and cellular survival. Although the zinc-regulated activities are similar throughout the gene networks, the specific genes that are affected vary significantly between different cell types, a situation that helps to elucidate the disparity of the effects that zinc has on different leukocyte populations.

Notes

Acknowledgments

We thank Ann Scarborough for technical assistance with the microarrays. This study was partially supported by DFG grant HA4318/3-2 and the EU project ZINCAGE (Food-CT-2003-506850).

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Copyright information

© Feinstein Institute for Medical Research 2007

Authors and Affiliations

  • Hajo Haase
    • 1
  • Dawn J. Mazzatti
    • 2
  • Andrew White
    • 3
  • Klaus H. Ibs
    • 1
  • Gabriela Engelhardt
    • 1
  • Silke Hebel
    • 1
  • Jonathan R. Powell
    • 2
  • Lothar Rink
    • 1
  1. 1.Institute of Immunology, University HospitalRWTH Aachen UniversityAachenGermany
  2. 2.Unilever Corporate ResearchSharnbrookUK
  3. 3.Unilever Measurement SciencesSharnbrookUK

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