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Molecular Medicine

, Volume 13, Issue 9–10, pp 527–541 | Cite as

DNA Microarrays: a Powerful Genomic Tool for Biomedical and Clinical Research

  • Victor Trevino
  • Francesco Falciani
  • Hugo A. Barrera-Saldaña
Research Article

Abstract

Among the many benefits of the Human Genome Project are new and powerful tools such as the genome-wide hybridization devices referred to as microarrays. Initially designed to measure gene transcriptional levels, microarray technologies are now used for comparing other genome features among individuals and their tissues and cells. Results provide valuable information on disease subcategories, disease prognosis, and treatment outcome. Likewise, they reveal differences in genetic makeup, regulatory mechanisms, and subtle variations and move us closer to the era of personalized medicine. To understand this powerful tool, its versatility, and how dramatically it is changing the molecular approach to biomedical and clinical research, this review describes the technology, its applications, a didactic step-by-step review of a typical microarray protocol, and a real experiment. Finally, it calls the attention of the medical community to the importance of integrating multidisciplinary teams to take advantage of this technology and its expanding applications that, in a slide, reveals our genetic inheritance and destiny.

Notes

Acknowledgments

HABS thanks the Staff of the Microarray Technology EMBO-INER Advanced Practical Course for enjoyable course lessons, materials and results; Peter Davies, Nancy and Greg Shipley of UT Medical School for additional laboratory training; Albert Sasson for critical reading of the manuscript and the offices of the Dean of his school and of the President of his University for support. Victor Trevino thanks Darwin Trust of Edinburgh and CONACyT for his PhD scholarship, and ITESM for support.

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Copyright information

© Feinstein Institute for Medical Research 2007

Authors and Affiliations

  • Victor Trevino
    • 1
    • 2
  • Francesco Falciani
    • 2
  • Hugo A. Barrera-Saldaña
    • 3
  1. 1.Instituto Tecnológico y de Estudios Superiores de MonterreyMonterreyMéxico
  2. 2.School of BiosciencesUniversity of BirminghamBirminghamUK
  3. 3.Departamento de Bioquímica, Facultad de Medicina de la Universidad Autónoma de Nuevo LeónLaboratorio de Genómica y Bioinformática del Unidad de Laboratorios de Ingeniería y Expresión GenéticaMonterreyMéxico

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