Molecular Medicine

, Volume 13, Issue 1–2, pp 69–78 | Cite as

Interplay of Genes Regulated by Estrogen and Diindolylmethane in Breast Cancer Cell Lines

  • Laura Mulvey
  • Alamelu Chandrasekaran
  • Kai Liu
  • Sarah Lombardi
  • Xue-Ping Wang
  • Karen J. Auborn
  • Leslie Goodwin
Research Article


Diindolylmethane (DIM), a biologically active congener of indole-3-carbinol (I3C) derived from cruciferous vegetables, is a promising agent for the prevention of estrogen-sensitive cancers. Both DIM and estrogen affect transcription of genes by binding receptors, such as aryl hydrocarbon receptor (AhR) or estrogen receptors (ER). Gene regulation by DIM and estradiol (E2) can be very complex. While DIM typically binds the AhR, this complex can directly associate with the ER, recruit co-activators that bind to estrogen-responsive promoters, and activate transcription. Alternately, DIM can bind the ER directly. In this study, we have analyzed gene expression using microarray profiling and quantitative real time-polymerase chain reaction in MCF7 breast cancer cells treated with E2 (1 nM) or DIM (25 µM) alone or in combination for 16 h. The interplay of E2 and DIM was reflected in the expression of a subset of genes (<90) in which the combination of E2 and DIM acted either additively or antagonistically to alter gene expression.



Laura Mulvey and Alamelu Chandrasekaran contributed equally to this work. This work was supported by RO1-CA733850 to KJA from the National Institutes of Health.


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Copyright information

© Feinstein Institute for Medical Research 2007

Authors and Affiliations

  • Laura Mulvey
    • 1
  • Alamelu Chandrasekaran
    • 1
  • Kai Liu
    • 1
  • Sarah Lombardi
    • 1
  • Xue-Ping Wang
    • 1
  • Karen J. Auborn
    • 1
    • 2
    • 3
  • Leslie Goodwin
    • 1
  1. 1.Feinstein Institute for Medical ResearchManhassetUSA
  2. 2.Department of Otolaryngology, Long Island Jewish Medical CenterThe Long Island Campus of Albert Einstein College of MedicineNew Hyde ParkUSA
  3. 3.Department of Microbiology and ImmunologyAlbert Einstein College of MedicineBronxUSA

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