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Molecular Medicine

, Volume 10, Issue 7–12, pp 104–111 | Cite as

Disease-Associated Prion Protein Elicits Immunoglobulin M Responses In Vivo

  • Mourad Tayebi
  • Perry Enever
  • Zahid Sattar
  • John Collinge
  • Simon Hawke
Articles

Abstract

Prion diseases such as Creutzfeldt-Jakob disease are believed to result from the misfolding of a widely expressed normal cellular prion protein, PrPc. The resulting disease-associated isoforms, PrPSc, have much higher β-sheet content, are insoluble in detergents, and acquire relative resistance to proteases. Although known to be highly aggregated and to form amyloid fibrils, the molecular architecture of PrPSc is poorly understood. To date, it has been impossible to elicit antibodies to native PrPSc that are capable of recognizing PrPSc without denaturation, even in Prn-Po/o mice that are intolerant of it. Here we demonstrate that antibodies for native PrPc and PrPSc can be produced by immunization of Prn-Po/o mice with partially purified PrPc and PrPSc adsorbed to immunomagnetic particles using high-affinity anti-PrP monoclonal antibodies (mAbs). Interestingly, the polyclonal response to PrPSc was predominantly of the immunoglobulin M (IgM) isotype, unlike the immunoglobulin G (IgG) responses elicited by PrPc or by recombinant PrP adsorbed or not to immunomagnetic particles, presumably reflecting the polymeric structure of disease-associated prion protein. Although heat-denatured PrPSc elicited more diverse antibodies with the revelation of C-terminal epitopes, remarkably, these were also predominantly IgM suggesting that the increasing immunogenicity, acquisition of protease sensitivity, and reduction in infectivity induced by heat are not associated with dissociation of the PrP molecules in the diseased-associated protein. Adsorbing native proteins to immunomagnetic particles may have general applicability for raising polyclonal or monoclonal antibodies to any native protein, without attempting laborious purification steps that might affect protein conformation.

Notes

Acknowledgments

We thank Ray Young for preparation of figures and the animal team at the Charing Cross prion facility. This work is supported by grants from the Medical Research Council (UK).

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Copyright information

© Feinstein Institute for Medical Research 2004

Authors and Affiliations

  • Mourad Tayebi
    • 1
    • 2
  • Perry Enever
    • 1
  • Zahid Sattar
    • 1
  • John Collinge
    • 1
    • 2
  • Simon Hawke
    • 1
    • 2
  1. 1.MRC Prion Unit and Department of Neurodegenerative Disease, Institute of NeurologyUniversity College LondonLondonUK
  2. 2.Department of Neurogenetics, Division of Neuroscience and Psychological Medicine, Faculty of MedicineImperial CollegeLondonUK

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