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Determination of a Biguanide, Metformin, by High-Performance Liquid Chromatography with Precolumn Fluorescence Derivatization

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Abstract

A sensitive method is described for determining a biguanide drug, metformin, by high-performance liquid chromatography with precolumn fluorescence derivatization. Metformin and phenylguanidine, as an internal standard, react with a novel fluorogenic reagent, desyl chloride, or benzoin to afford the corresponding fluorescent derivatives, which are separated by reversed-phase chromatography on a TSKgel ODS-120T column with linear-gradient elution. The applicability of the method to the determination of metformin in human serum is also demonstrated. The calibration curve of metformin was linear over the range of 0–100 nmol ml-1 in human serum. The detection limit (S/N=3) was 1.3 pmol per 20-μl injection volume.

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Authors and Affiliations

  1. Daiichi College of Pharmaceutical Sciences, Tamagawa, Minami, Fukuoka, 815, Japan

    Mutsuko Ohta & Masatake Iwasaki

  2. Faculty of Phamaceutical Sciences, Kyushu Uviversity, 62, Maidashi, Higashi, Fukuoka, 812, Japan

    Masaaki Kai & Yosuke Ohkura

Authors
  1. Mutsuko Ohta
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  2. Masatake Iwasaki
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  3. Masaaki Kai
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  4. Yosuke Ohkura
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Ohta, M., Iwasaki, M., Kai, M. et al. Determination of a Biguanide, Metformin, by High-Performance Liquid Chromatography with Precolumn Fluorescence Derivatization. ANAL. SCI. 9, 217–220 (1993). https://doi.org/10.2116/analsci.9.217

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  • DOI: https://doi.org/10.2116/analsci.9.217

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