Abstract
Eight novel 2-chloro-4-nitropheny] maltotetraosides modified at the 64- and/or 44-position were synthesized for human pancreatic and salivary a-amylase substrates. In these compounds, 2-chloro-4-nitrophenyl O-[4,6-di-O-(N-isopro-pyl)carbamoyl-α-d-glucopyranosyl]-(l→4)-bis[O-α-d-glucopyranosyl-(1→4)]-β-d-glucopyranoside (9) was rapidly hydrolyzed by the two amylases at a limited position to produce 2-chloro-4-nitrophenyl β-d-glucopyranoside. Taking advantage of the properties, 9 was found to be a good substrate for a human a-amylase assay using only β-glucosidase as a coupled enzyme.
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Tokutake, S., Saito, S. & Yamaji, N. Terminal Modified 2-Chloro-4-nitrophenyl β-d-Maltotetraosides as Substrates for a Human α-Amylase Assay. ANAL. SCI. 8, 669–674 (1992). https://doi.org/10.2116/analsci.8.669
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DOI: https://doi.org/10.2116/analsci.8.669