Abstract
The sensitivity for the chemiluminescent (CL) detection of nucleosides and their derivatives was improved by using clinoptirolite chemically modified with cupric nitrate, Cu-Zeo. Cu-Zeo plays a key role as a solid medium for such so-called material exchangers as ion exchange between copper(II) coated on Cu-Zeo and nucleosides and their derivatives. The properties for material exchange were examined by a batch method in which Cu-Zeo (1.5 g; particle size, 250 mesh) was added into the analyte solution (100 ml). Analytes were indirectly determined by the CL analysis of cupric ions eluted from the Cu-Zeo through the material exchange. For the CL detection of cupric ions, an FIA/CL system using a 1,10-phenanthroline CL was employed. The lower limits of detection for analytes were improved by 42 times for adenosine (1.9 pmol/20 μl) and by 16 times for uridine-5’-diphosphate (5.5 pmol/ 20 μl), compared with those by our previous method.
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Ohno, Y., Hobo, T., Tamura, R. et al. Sensitive Chemiluminescent Detection of Nucleosides and Their Derivatives Using the Material Exchange of Chemically Copper(II)-Modified Natural Zeolite. ANAL. SCI. 8, 647–653 (1992). https://doi.org/10.2116/analsci.8.647
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DOI: https://doi.org/10.2116/analsci.8.647