Abstract
New labeling agent, 2-[2-(azidocarbonyl)ethyl]-3-methyl-1,4-naphthoquinone (AMQ), has been synthesized and developed for the analysis of alcohols in HPLC with fluorescence or electrochemical detection after post column reduction with platinum catalyst. Optimal reaction conditions of AMQ were examined with 1-eicosanol selected as a model compound of alcohols. Alcohols were reacted with the reagent in acetone at 100°C for 15 min to form the corresponding carbamic acid esters, which were separated on an Inertsil C8 reversed-phase column with aqueous methanol containing 0.05M sodium Perchlorate. The detection limit for 1-eicosanol with electrochemical detection was 16 fmol/10µl injection volume.
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Nakajima, M., Wakabayashi, H., Yamato, S. et al. New Labeling Agent for High Performance Liquid Chromatographic Determination of Alcohols with Fluorescence and Electrochemical Detection. ANAL. SCI. 7 (Suppl 1), 173–176 (1991). https://doi.org/10.2116/analsci.7.Supple_173
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DOI: https://doi.org/10.2116/analsci.7.Supple_173