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A New Ceramic Type Spherical Fluoroapatite Beads for High Performance Liquid Chromatography of Natural Proteins

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Abstract

The chromatographical behaviors of proteins on FAP column are described. Firstly the elution patterns from FAP column were aproximately same as those from HAP column. The recovery estimated with cytochrome с and others were aproximately 100 % but the less recovery was estimated for denatured proteins. The pH dependancy of protein adsorption on FAP was determined. All of proteins including very acidic proteins were adsorbed at pH 5 and were eluted at pH 8.

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Authors and Affiliations

  1. Department of Chemistry, Tokyo Metropolitan University, Faculty of Science, Setagaya-ku, Tokyo, 158, Japan

    Toshiaki Sato, Kazuyuki Ohuchi, Ichirou Kohno & Tsuneo Okuyama

  2. Asahi Optical Co. Ltd., Itabashi-ku, Tokyo, 174, Japan

    Satoshi Fujinuma, Kazuhiko Naganuma & Tetsuo Ogawa

Authors
  1. Toshiaki Sato
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  2. Kazuyuki Ohuchi
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  3. Ichirou Kohno
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  4. Satoshi Fujinuma
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  5. Kazuhiko Naganuma
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  6. Tetsuo Ogawa
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  7. Tsuneo Okuyama
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Sato, T., Ohuchi, K., Kohno, I. et al. A New Ceramic Type Spherical Fluoroapatite Beads for High Performance Liquid Chromatography of Natural Proteins. ANAL. SCI. 7 (Suppl 1), 143–146 (1991). https://doi.org/10.2116/analsci.7.Supple_143

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  • DOI: https://doi.org/10.2116/analsci.7.Supple_143

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