Skip to main content
SpringerLink
Log in

Determination of Monophosphate-Type Nucleotides by High-Performance Liquid Chromatography with Phosphorus-Selective Detection

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

A selective, method for the determination of monophosphate-type nucleotides based on the molybdenum blue method has been developed. The monophosphates were decomposed to produce the orthophosphate-molybdenum blue complex by heating at 140 °C without any oxidizing agent, while di- or triphosphates hardly produced the complex under the same condition. The separation of monophosphates was carried out in the ion-exchange mode, and the detection limits were 25 ppb as P for the monophosphates examined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. W. Hu, H. Haraguchi and T. Takeuchi, J. Chromatogr. in press.

  2. Japanese Industrial Standards Committee, Testing Methods for Industrial Water JIS K 0102-1986. Japanese Standards Committee Association, Tokyo, 1986, P.158.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Applied Chemistry, School of Engineering, Nagoya University, Chikusa-ku, Nagoya, 464-01, Japan

    Wenzhi Hu & Hiroki Haraguchi

  2. Research Center for Resource and Energy Conservation, Nagoya University, Chikusa-ku, Nagoya, 464-01, Japan

    Toyohide Takeuchi & Hiroki Haraguchi

Authors
  1. Wenzhi Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Toyohide Takeuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiroki Haraguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hu, W., Takeuchi, T. & Haraguchi, H. Determination of Monophosphate-Type Nucleotides by High-Performance Liquid Chromatography with Phosphorus-Selective Detection. ANAL. SCI. 7 (Suppl 1), 135–138 (1991). https://doi.org/10.2116/analsci.7.Supple_135

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.7.Supple_135

Keywords

Search

Navigation