Abstract
A high-performance liquid chromatographic method was developed for the assay of ofloxacin in serum. The serum samples were filtered through a Molcut II membrane filter after partial deproteinization in order to remove proteins. The filtrate was preseparated on a reversed-phase precolumn and introduced onto a reversed-phase analytical column by applying column-switching techniques. Ofloxacin and nalidixic acid as internal standards were detected by absorbance measurements at 300 nm. Using this system the concentration of ofloxacin could be determined at a limit of the order of 20 ng/ ml, with a linear dynamic range of 80 - 4000 ng/ ml. This procedure was applied to pharmacokinetic studies of healthy volunteers treated with ofloxacin.
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Ohkubo, T., Kudo, M. & Sugawara, K. High-Performance Liquid Chromatographic Determination of Ofloxacin in Serum. ANAL. SCI. 7, 741–743 (1991). https://doi.org/10.2116/analsci.7.741
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DOI: https://doi.org/10.2116/analsci.7.741