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High-Performance Liquid Chromatographic Determination of Pyrroloquinoline Quinone as Acetone Adduct

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Abstract

A high performance liquid chromatographic method for the determination of pyrroloquinoline quinone (PQQ) is described. PQQ reacts readily with acetone in a weakly alkaline medium, giving a stable adduct, 5-acetonyl PQQ. The adduct was separated on a Develosil C18-5 column using a mixture of methanol and 0.06 M phosphoric acid, and was detected at 254 nm. The response was linear over the range (1 - 20)×10-6 M of PQQ and the detection limit was 2 pmol for a 20-μl injection volume.

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Authors and Affiliations

  1. Gifu Pharmaceutical University, Mitahora-higashi, Gifu, 502, Japan

    Kenji Kano, Bunji Uno, Chie Kawasaki, Kiyomi Horiki & Satoshi Kawai

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  1. Kenji Kano
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  2. Bunji Uno
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  3. Chie Kawasaki
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  4. Kiyomi Horiki
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  5. Satoshi Kawai
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Kano, K., Uno, B., Kawasaki, C. et al. High-Performance Liquid Chromatographic Determination of Pyrroloquinoline Quinone as Acetone Adduct. ANAL. SCI. 7, 737–739 (1991). https://doi.org/10.2116/analsci.7.737

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  • DOI: https://doi.org/10.2116/analsci.7.737

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