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Polarized Photometric Detector for High-Performance Liquid Chromatography

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Abstract

A photometric detector for an optically active compound is described in which two polarizers are set on both sides of the flow cell. When a nonchromophoric, optically active compound passes through the cell, the intensity of natural light transmitted through the polarizers and flow cell is changed. The detection of such a sample is possible photometrically. A large inclination angle between the polarizers increases the absorbance change and allows dextrorotatory and levorotatory to be differentiated. The direct HPLC/ photometric detection of sugars is presented as an example of this device.

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Authors and Affiliations

  1. Toyama Institute of Health, Nakataikoyama, Kosugi-maehi, Toyama, 939-03, Japan

    Atsushi Yamamoto, Akinobu Matsunaga & Eiichi Mizukami

  2. Faculty of Pharmaceutical Sciences, Kanazawa University, Takara-machi, Kanazawa, 920, Japan

    Kazuichi Hayakawa & Motoichi Miyazaki

Authors
  1. Atsushi Yamamoto
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  2. Akinobu Matsunaga
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  3. Kazuichi Hayakawa
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  4. Eiichi Mizukami
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  5. Motoichi Miyazaki
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Yamamoto, A., Matsunaga, A., Hayakawa, K. et al. Polarized Photometric Detector for High-Performance Liquid Chromatography. ANAL. SCI. 7, 719–721 (1991). https://doi.org/10.2116/analsci.7.719

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  • DOI: https://doi.org/10.2116/analsci.7.719

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