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Purification of Food Blue No. 1 by Rotation Locular Countercurrent Chromatography

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Abstract

The purification of Food Blue No. 1 was examined using rotation locular countercurrent chromatography, which uses the different partition between two immiscible liquids for the chromatographic purification of various substances. Commercially available reagent-grade Food Blue No, 1 allowed the presence of ca. 92% of the main dye and many kinds of subsidiary colors by high performance liquid chromatography. The phase system used for purification comprised 1-butanol-water (1:1 by volume). Purified Blue No. 1 (ca. 220 mg) was obtained in a proportion 99.7% higher from ca. 2 g of the commercial dye.

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Authors and Affiliations

  1. College of Pharmacy, Nihon University, Narashinodai, Fimabashi, 274, Japan

    Yozo Kabasawa & Kazufusa Shinomiya

  2. Institute of Public Health, Shiroganedai, Tokyo, 106, Japan

    Hiroyuki Nakazawa & Masahiko Fujita

  3. Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University, Sugitani, Toyama, 930-01, Japan

    Takenori Tanimura

Authors
  1. Yozo Kabasawa
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  2. Kazufusa Shinomiya
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  3. Hiroyuki Nakazawa
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  4. Takenori Tanimura
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  5. Masahiko Fujita
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Kabasawa, Y., Shinomiya, K., Nakazawa, H. et al. Purification of Food Blue No. 1 by Rotation Locular Countercurrent Chromatography. ANAL. SCI. 7, 273–276 (1991). https://doi.org/10.2116/analsci.7.273

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  • DOI: https://doi.org/10.2116/analsci.7.273

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