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Application of Thermospray Liquid Chromatography /Mass Spectrometry to the Analysis of Water-Soluble Vitamins

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Abstract

Thermospray liquid chromatography/mass spectrometry with a new, strongly acidic mobile phase, i.e., formic acid-ammonium formate buffer system, was applied to the analysis of five kinds of water-soluble vitamins. Their detection limits, linearity and reproducibilities were examined. The mass spectra of the individual vitamins gave MH* ions; some of these showed fragment ions giving information on molecular structure. Detection limits (S/ N>10) were 10–100 pg; reproducibility of 1.2–4.9% (RSD) was obtained for 10 μl syringe injections, based on peak areas. The water-soluble vitamins in a commercial health drink and in fermented soybean paste miso were determined by this method.

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Authors and Affiliations

  1. Analytical Applications Department, Shimadzu Corporation, Nishinokyo-kuwabaracho, Kyoto, 604, Japan

    Junko Iida & Takeshi Murata

Authors
  1. Junko Iida
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  2. Takeshi Murata
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Iida, J., Murata, T. Application of Thermospray Liquid Chromatography /Mass Spectrometry to the Analysis of Water-Soluble Vitamins. ANAL. SCI. 6, 273–276 (1990). https://doi.org/10.2116/analsci.6.273

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  • DOI: https://doi.org/10.2116/analsci.6.273

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