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Liquid Chromatography of Basic Solutes on Silica Gel and Aluminum Oxide

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Abstract

Chromatography of basic solutes has been described on non-modified silica and alumina using aqueous buffers with suitable modifiers. On these packing materials selectivity and retention of the compounds are influenced in various ways; by the modifier content, the electrolyte concentration and the pH. Generally, only cationic species are retained on these sorbents. Their retention decreases with increasing electrolyte concentration. The relationship between retention and modifier content is more complicated and, moreover, different for the two packing materials. The differences in selectivity will be illustrated by comparing the chromatography of combinations of basic drugs (e.g. phenylethytamines, alkaloids), the analysis of basic drugs in pharmaceutical formulations (e.g. prednisolone, physostigmine) and the detection of basic drug metabolites (e.g. amphoteric glucuronides) on silica and alumina. The aqueous nature of the eluent allows the combination of these packing materials and the use of column switching systems.

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, Faculty of Pharmacy, State University of Utrecht, Catharijnesingel 60, 3511 GH, Utrecht, The Netherlands

    U. K. Underberg-Chitoe & W. J. M. Underberg

  2. Division of Analytical Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden University, P. O. Box 9502, 2300 RA, Leiden, The Netherlands

    H. Lingeman

Authors
  1. U. K. Underberg-Chitoe
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  2. W. J. M. Underberg
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  3. H. Lingeman
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Underberg-Chitoe, U.K., Underberg, W.J.M. & Lingeman, H. Liquid Chromatography of Basic Solutes on Silica Gel and Aluminum Oxide. ANAL. SCI. 4, 91–96 (1988). https://doi.org/10.2116/analsci.4.91

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  • DOI: https://doi.org/10.2116/analsci.4.91

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