Skip to main content
SpringerLink
Log in

Determination of Sub-nmol Hydrogen Peroxide by Electrochemiluminescence of Luminol in Aqueous Solution

  • Original Paper
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

An electrochemiluminescence method was developed for determination of sub-nmol hydrogen peroxide in aqueous solution. Luminol was electrochemically oxidized, then reacted with hydrogen peroxide; it emitted light of the maximum wavelength of 436 nm with a half-life of less than 0.2 s from the electrode surface. In flow injection analysis a carrier solution (phosphate buffer, pH 7–9) containing 17 μM luminol was delivered at the flow rate of 0.5 ml/min. The luminescence was detected by a photon counter in a spiral cell under the applied potential of 0.7 V vs. SCE. A straight line calibration plot was obtained with good reproducibility [RSD 1.6% (n=5)] in the concentration range of 0.066 to 0.6 nmol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. Blatchford and D. J. Malcome-Lawes, J. Chromatogr., 321, 227 (1985).

    Article  CAS  Google Scholar 

  2. C. Blatchford, E. Humphreys and D. J. Malcome-Lawes, J. Chromatogr., 329, 281 (1985).

    Article  CAS  Google Scholar 

  3. H. Imai, H. Yoshida, T. Masujima and M. Yanagitani, Bunseki Kagaku, 30, 419 (1981).

    Article  CAS  Google Scholar 

  4. H. Hwang and P. K. Dasgupta, Anal. Chem., 58, 1521 (1986).

    Article  CAS  Google Scholar 

  5. T. P. Whitehead, G. H. G. Thorpe, T. J. N. Carter, C. Groucult and L. J. Kricka, Nature [London], 305, 158 (1983).

    Article  CAS  Google Scholar 

  6. M. Tabata, C. Fukunaga, M. Ohyabu and T. Murachi, J. Appl. Biochem., 6, 251 (1984).

    CAS  PubMed  Google Scholar 

  7. M. Maeda and A. Tsuji, Anal. Chim. Acta, 167, 241 (1985).

    Article  CAS  Google Scholar 

  8. H. Imai, H. Yoshida, T. Masujima and T. Owa, Bunseki Kagaku, 33, 54 (1984).

    Article  CAS  Google Scholar 

  9. T. Owa, T. Masujima, H. Yoshida and H. Imai, Bunseki Kagaku, 33, 568 (1984).

    Article  CAS  Google Scholar 

  10. K. E. Haapakka and J. J. Kankare, Anal. Chim. Acta, 118, 333, (1980).

    Article  CAS  Google Scholar 

  11. I. Kamiya, “Chemiluminescence”, (Kagakuhakkou, in Japanese) p. 55, Kodansha, Tokyo (1972).

    Google Scholar 

  12. P. B. Shevlin and H. A. Newfeld, J. Org. Chem., 35, 2178 (1970).

    Article  CAS  Google Scholar 

  13. M. M. Rauhut, A. M. Semsel and B. G. Roberts, J. Org. Chem., 31, 2431 (1966).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Fukuyama University, Fukuyama, 729-02, Japan

    Sachiko Sakura & Hideo Imai

Authors
  1. Sachiko Sakura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hideo Imai
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sakura, S., Imai, H. Determination of Sub-nmol Hydrogen Peroxide by Electrochemiluminescence of Luminol in Aqueous Solution. ANAL. SCI. 4, 9–12 (1988). https://doi.org/10.2116/analsci.4.9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.4.9

Keywords

Search

Navigation