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Fluorometric Determination of Uric Acid with 4,5-Dimethyl-o-phenylenediamine

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Abstract

A new sensitive fluorometric method for the determination of uric acid is presented. Uric acid reacted with 4,5-dimethyl-o-phenylenediamine (DMPD) after the oxidation using /V-bromosuccinimide (NBS) to give a green fluorescence with excitation maxima at 350 nm and 400 nm, and an emission maximum at 500 nm. By the use of the fluorescence reaction, uric acid in aqueous solution was determined in the range of 0.01 -5.0 μg ml-1, and the relative standard deviation for 1.0 μg ml-1 uric acid was 0.51 % (n=10). The proposed method could be applied to the determination of uric acid in serum.

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Authors and Affiliations

  1. Meiji College of Pharmacy, Nozawa, Setagaya, Tokyo, 154, Japan

    Masatoki Katayama, Yoshio Mukai & Hirokazu Taniguchi

Authors
  1. Masatoki Katayama
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  2. Yoshio Mukai
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  3. Hirokazu Taniguchi
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Katayama, M., Mukai, Y. & Taniguchi, H. Fluorometric Determination of Uric Acid with 4,5-Dimethyl-o-phenylenediamine. ANAL. SCI. 4, 587–590 (1988). https://doi.org/10.2116/analsci.4.587

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  • DOI: https://doi.org/10.2116/analsci.4.587

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