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Enzyme Electrode for Determination of Glycerophosphate by Combined Use of Glycerophosphate Oxidase and Peroxidase

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Abstract

An enzyme sensor for L-α-glycerophosphate was prepared by covalent fixation of glycerophosphate oxidase and peroxidase by use of bovine serum albumin and glutaraldehyde onto a glassy carbon electrode silanized with 3-aminopropyl triethoxysilane. The sensor showed good linear current responses in the range of 5 to 100 ώM glycerophosphate (γ=0.999, n=7); the response time was about 20 s. The sensor was stable for at least 60 d. Sugar phosphates did not have any appreciable effect on the response of the sensor, but reducing materials such as L-ascorbic acid and L-cysteine gave some errors that changed with concentrations.

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Authors and Affiliations

  1. Development Division, Horiba, Lid., Kissyoin, Minami, Kyoto, 601, Japan

    Junji Kojima

  2. Laboratory of Food Chemistry, College of Agriculture, University of Osaka Prefecture, Mozu-Umemachi, Sakai, 591, Japan

    Naofumi Morita & Masanosuke Takagi

Authors
  1. Junji Kojima
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  2. Naofumi Morita
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  3. Masanosuke Takagi
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Kojima, J., Morita, N. & Takagi, M. Enzyme Electrode for Determination of Glycerophosphate by Combined Use of Glycerophosphate Oxidase and Peroxidase. ANAL. SCI. 4, 497–500 (1988). https://doi.org/10.2116/analsci.4.497

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  • DOI: https://doi.org/10.2116/analsci.4.497

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