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A Flow Injection Technique for Measurement of Penicillinase Activity

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Abstract

A facile technique was developed for the determination of penicillinase activity based on flow injection analysis (FIA). The analytical condition of the method utilizing a substrate (benzylpenicillin) solution as the carrier was optimized. Penicillinase solution was injected into the FIA system where the substrate was undergone enzymatic hydrolysis to form penicilloate. The decrease in the substrate was monitored at 229 nm. A linear correlation was observed between a conventional photometric method and the present FIA method in the range of 0.04 to 6.6 unit ml-1. The relative standard deviations (n=18) for the determination of penicillinase at 0.07, 0.5 and 2.8 unit ml-1 were 0.65, 0.95 and 2.9%, respectively. The detection limit was 0.03 unit ml-1 with a sample size of 5 ώl. The FIA method was applied to the examination of penicillinase stability in solutions.

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Authors and Affiliations

  1. Laboratory of Beecham Yakuhin Co., Ltd., Bubai, Fuchu, Tokyo, 183, Japan

    Shinobu Kudoh

  2. Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo, Hongo, Tokyo, 113, Japan

    Hiroshi Nakamura

Authors
  1. Shinobu Kudoh
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  2. Hiroshi Nakamura
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Kudoh, S., Nakamura, H. A Flow Injection Technique for Measurement of Penicillinase Activity. ANAL. SCI. 4, 493–495 (1988). https://doi.org/10.2116/analsci.4.493

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  • DOI: https://doi.org/10.2116/analsci.4.493

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