Abstract
Polyaromatic compounds which possess different base-pair selectivities in their DNA-binding (intercalating) properties were covalently bound to polyethylene glycol (PEG) via ester bonds. These PEG-bound intercalators are highly soluble in water and induced the retardation of DNA electrophoresis in agarose gel. The pattern of mobility retardation reflected the binding characteristics of the parent intercalators to DNA. Use of PEG-bound intercalators in the gel electrophoresis is discussed as a simple and convenient method for the characterization of polyaromatic DNA intercalators of low water solubility.
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References and Notes
Alternating AT sequence: (AT), sequence in the DNA chain. Nonalternating AT sequence: a sequence consisting of A and T; A and T do not appear in an alternating manner.
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Takenaka, S., Aoyagi, Y., Dohtsu, K. et al. Simple Characterization of DNA Intercalators by Retarded Gel Electrophoresis. ANAL. SCI. 4, 481–486 (1988). https://doi.org/10.2116/analsci.4.481
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DOI: https://doi.org/10.2116/analsci.4.481