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Electrochemical Detector Using Carbon Fiber Working Electrode for Micro High Performance Liquid Chromatography

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Abstract

A tubular electrolytic cell of ultra micro volume, made of a single carbon fiber (7 μm o.d., 15 mm length) as the working electrode material and a fused silica tube (50 μm i.d.) as the flow cell part, was designed for use as the electrochemical detector in micro high performance liquid chromatography (micro HPLC). The effective cell volume is about 0.03 μl. This detector with carbon fiber was useful for detection of catecholamines and their metabolites. Their detection limits were about 1–2 pg and the linear dynamic range was about 103. The detector was successfully applied to urinary catecholamine analysis by micro HPLC and to rapid detection of electroactive species in fast micro HPLC.

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References

  1. M. V. Novotny and D. Ishii (eds.), “Microcolumn Separations: Columns, Instrumentation and Ancillary Techniques”, Elsevier Scientific Publishers B. V., Amsterdam (1985).

    Google Scholar 

  2. Y. Hirata, P. T. Lin, M. Novotny and R. M. Wightman, J. Chromatogr. 181, 287 (1980).

    Article  CAS  Google Scholar 

  3. M. Goto, Y. Koyanagi and D. Ishii, J. Chromatogr., 208, 261 (1981).

    Article  CAS  Google Scholar 

  4. M. Goto, T. Nakamura and D. Ishii, J. Chromatogr., 226, 33, (1981).

    Article  CAS  Google Scholar 

  5. M. Goto, E. Sakurai and D. Ishii, J. Chromatogr., 238, 357 (1982).

    Article  CAS  Google Scholar 

  6. M. Goto, Z. Gongwei and D. Ishii, J. Chromatogr., 275, 271 (1983).

    Article  CAS  Google Scholar 

  7. J. F. Ponchon, R. Cespuglio, F. Gonon, M. Jouvet and J. F. Pujol, Anal. Chem., 51, 1483 (1979).

    Article  CAS  Google Scholar 

  8. F. G. Gonon, C. M. Fombarlet, M. J. Buda and J. F. Pujol, Anal. Chem., 53, 1386 (1981).

    Article  CAS  Google Scholar 

  9. R. M. Wightman, Anal. Chem., 53, 1125A (1981).

    Article  CAS  Google Scholar 

  10. M. Ikeda, H. Miyazaki, N. Mugitani and A. Matsushita, Nurosci. Res., 1, 171 (1984).

    Article  CAS  Google Scholar 

  11. M. Goto, in “Quantitative analysis of catecholamines and related compounds”, pp. 187–208, ed. A. M. Krstulovic, Ellis Horwood Limited, Chichester, England (1986).

  12. K. Aoki, K. Honda, K. Tokuda and H. Matsuda, J. Electroanal. Chem. Interfacial Electrochem., 182, 267 (1985).

    Article  CAS  Google Scholar 

  13. T. Kakutani, H. Miyazaki and M. Ikeda, Bunseki, 1986, 778.

    Google Scholar 

  14. L. A. Knecht, E. J. Guthrie and J. W. Jorgenson, Anal. Chem., 56, 479 (1984).

    Article  CAS  Google Scholar 

  15. R. L. St. Claire III and J. W. Jorgenson, J. Chromatogr. Sci., 23, 186 (1985).

    Article  Google Scholar 

  16. J. G. White, R. L. St. Claire III and J. W. Jorgenson, Anal. Chem., 58, 293 (1986).

    Article  CAS  Google Scholar 

  17. M. Goto and K. Shimada, Chromatographia, 21, 631 (1986).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Center for Resource and Energy Conservation, Nagoya University, Nagoya, 464, Japan

    Masashi Goto

  2. Department of Applied Chemistry, Faculty of Engineering, Nagoya University, Nagoya, 464, Japan

    Ken Shimada, Toyohide Takeuchi & Daido Ishii

Authors
  1. Masashi Goto
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  2. Ken Shimada
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  3. Toyohide Takeuchi
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  4. Daido Ishii
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Goto, M., Shimada, K., Takeuchi, T. et al. Electrochemical Detector Using Carbon Fiber Working Electrode for Micro High Performance Liquid Chromatography. ANAL. SCI. 4, 17–20 (1988). https://doi.org/10.2116/analsci.4.17

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  • DOI: https://doi.org/10.2116/analsci.4.17

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