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Determination of S-Sulfocysteine and S-Sulfoglutathione in Plasma and Red Blood Cells by High Performance Liquid Chromatography

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Abstract

A simultaneous fluorometric determination method of S-sulfocysteine and S-sulfoglutathione was established by HPLC using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) as a prelabeling reagent. Chromatographic conditions were as follows: Column, TSK gel NH2-60 (150 mm×4 mm i.d.); eluent, 0.1 M citrate buffer (pH 3.0) containing 10% methanol; flow rate, 1.0 ml/min; sample size, 100 μl; detection, Ex 475, Em 545 nm. Calibration curves for S-sulfocysteine and S-sulfoglutathione were linear over the range of 0.1 to 10 nmol. S-Sulfoglutathione was identified as an endogenous compound in rabbit red blood cells by the use of the present method. This method was also applied to the determination of S-sulfocysteine and S-sulfoglutathione in rabbit plasma and red blood cells after injection of sodium bisulfite.

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  1. Faculty of Pharmaceutical Sciences, Chiba University, Yayoicho, Chiba, 260, Japan

    Tadayasu Togawa, Masahiro Kato, Noriko Nagai & Toshio Imanari

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  1. Tadayasu Togawa
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  2. Masahiro Kato
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  3. Noriko Nagai
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  4. Toshio Imanari
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Togawa, T., Kato, M., Nagai, N. et al. Determination of S-Sulfocysteine and S-Sulfoglutathione in Plasma and Red Blood Cells by High Performance Liquid Chromatography. ANAL. SCI. 4, 101–104 (1988). https://doi.org/10.2116/analsci.4.101

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  • DOI: https://doi.org/10.2116/analsci.4.101

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