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Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection

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Abstract

An analytical method was developed and validated for the measurement of hydroxyproline (Hyp) levels in mouse kidney by high-performance liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) using an analytical column specially designed for the LC/MS/MS analysis for intact amino acids. Tissue hydrolyzed with hydrochloric acid could be directly injected into the LC/MS/MS, as well as separated and detected using the deuterium labelled Hyp as an internal standard. The calibration curve showed good linearity from 5 to 500 nmol/mg of tissue; the precision and accuracy, including within- and between-run, were less than 6% and within 100 ± 6%, respectively.

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Acknowledgments

We thank Takayuki Kuroda, Shiho Konishi, Norito Yoshimura, and Risa Yamanaka for the preparing the mice kidney samples.

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Authors and Affiliations

  1. Shionogi TechnoAdvance Research Co., Ltd., 3-1-1 Futaba-cho Toyonaka, Osaka, 561-0825, Japan

    Rika Shiota, Hirotoshi Morita, Tomoko Matsumoto & Atsushi Morimoto

  2. Biomarker R&D Department, Shionogi & Co. Ltd., 3-1-1 Futaba-cho, Toyonaka, Osaka, 561-0825, Japan

    Jun Hayakawa

  3. Pharmaceutical Research Division, Shionogi & Co. Ltd, 3-1-1 Futaba-cho, Toyonaka, Osaka, 561-0825, Japan

    Masako Oka & Hiroshi Kamimori

Authors
  1. Rika Shiota
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  2. Hirotoshi Morita
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  3. Tomoko Matsumoto
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  4. Atsushi Morimoto
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  5. Jun Hayakawa
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  6. Masako Oka
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  7. Hiroshi Kamimori
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Corresponding author

Correspondence to Hiroshi Kamimori.

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Shiota, R., Morita, H., Matsumoto, T. et al. Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection. ANAL. SCI. 33, 719–722 (2017). https://doi.org/10.2116/analsci.33.719

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  • DOI: https://doi.org/10.2116/analsci.33.719

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