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Effect of the Chain Length of a Modified Layer and Surface Roughness of an Electrode on Impedimetric Immunosensors

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Abstract

An ultrasensitive label-free impedimetric immunosensor is constructed by modifying a 3-mercaptoproponic acid (MPA) monolayer on highly rough gold nanostructure (AuNS)-electrodeposited screen printed carbon electrodes (SPCEs) for the detection of small molecular weight drugs (SMWDs), such as salbutamol (SAL). The SPCEs preoxidized in a 0.1 M H2SO4 solution (called po-SPCEH2SO4) are electrodeposited with the AuNS to increase the roughness factor to 23.64 ± 1.76, larger than the AuNS/po-SPCENaOH or the AuNS/po-SPCE{ in =PBS}. Furthermore, the MPA modified layer as a link for the anti-SAL immobilization to give the immunosensors an impedimetric signal-to-noise ratio larger than the 11-mercaptoundecanoic acid- and 16-mercaptohexadecanoic acid-modified layer, due to the lower interfacial impedance of the MPA monolayer. The MPA/AuNS/po-SPCEH2SO4-based immunosensors have a wide linear range of 1 fg mL−1 to 1 ng mL−1 and a limit of detection of 0.6 fg mL−1. Moreover, the immunosensors can practically quantify the SAL concentrations in 1000 times-diluted serum samples with good recovery.

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Authors and Affiliations

  1. Department of Bio-industrial Mechatronics Engineering, National Chung Hsing University, Taichung, Taiwan, 402, China

    Chia-Hung Lin, Ming-Jie Lin & Ching-Chou Wu

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  1. Chia-Hung Lin
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  2. Ming-Jie Lin
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  3. Ching-Chou Wu
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Correspondence to Ching-Chou Wu.

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Lin, CH., Lin, MJ. & Wu, CC. Effect of the Chain Length of a Modified Layer and Surface Roughness of an Electrode on Impedimetric Immunosensors. ANAL. SCI. 33, 327–331 (2017). https://doi.org/10.2116/analsci.33.327

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  • DOI: https://doi.org/10.2116/analsci.33.327

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