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Sequential Injection Chromatography with an Ultra-short Monolithic Column for the Low-Pressure Separation of α-Tocopherol and γ-Oryzanol in Vegetable Oils and Nutrition Supplements

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Abstract

A low-pressure separation procedure of α-tocopherol and γ-oryzanol was developed based on a sequential injection chromatography (SIC) system coupled with an ultra-short (5 mm) C-18 monolithic column, as a lower cost and more compact alternative to the HPLC system. A green sample preparation, dilution with a small amount of hexane followed by liquid–liquid extraction with 80% ethanol, was proposed. Very good separation resolution (Rs = 3.26), a satisfactory separation time (10 min) and a total run time including column equilibration (16 min) were achieved. The linear working range was found to be 0.4 - 40 μg with R2 being more than 0.99. The detection limits of both analytes were 0.28 μg with the repeatability within 5% RSD (n = 7). Quantitative analyses of the two analytes in vegetable oil and nutrition supplement samples, using the proposed SIC method, agree well with the results from HPLC.

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Authors and Affiliations

  1. Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Sujitra Thaithet & Somchai Lapanantnoppakhun

  2. Department of Chemistry, Xavier University, 3800 Victory Parkway, Cincinnati, OH, 45207, USA

    Supaporn Kradtap Hartwell

Authors
  1. Sujitra Thaithet
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  2. Supaporn Kradtap Hartwell
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  3. Somchai Lapanantnoppakhun
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Thaithet, S., Kradtap Hartwell, S. & Lapanantnoppakhun, S. Sequential Injection Chromatography with an Ultra-short Monolithic Column for the Low-Pressure Separation of α-Tocopherol and γ-Oryzanol in Vegetable Oils and Nutrition Supplements. ANAL. SCI. 33, 313–317 (2017). https://doi.org/10.2116/analsci.33.313

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  • DOI: https://doi.org/10.2116/analsci.33.313

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