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Hairpin-shaped DNA Templated Copper Na nopar tides for Fluorescence Detection of Adenosine Triphosphate Based on Ligation-mediated Exonuclease Cleavage

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Abstract

DNA-templated copper nanoparticles (CuNPs) have recently received considerable interest as functional fluorescent probes for biochemical analysis. In this work, a novel ATP-dependent ligation reactions (ATP-DLR) based ATP assay strategy was proposed by using hairpin-shaped (HS) DNA templated CuNPs as a fluorescent probe. Nick sealing by T4 DNA ligase leads to the formation of intact HS DNA, which can resist the exonuclease cleavage and be taken as the template for CuNPs formation, resulting in strong fluorescence. The proposed ATP detection is label free, sensitive and highly selective, and it has good linearity from 0.02 to 4 μM and a detection limit of 7 nM. This strategy is expected to promote the exploitation and application of DNA-templated CuNPs in biochemical and biomedical studies, and holds great promise in fluorescence detection for other ligation-related biomolecules.

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Acknowledgments

This work was supported by the Henan Province Foundation and Advanced Technology Research Program (No. 142300410348), the Research Start Funds Sponsored Program of Zhoukou Normal University (ZKNUB2013002), the Natural Science Basic Research Project of the Education Department of Henan Province (Grant No. 17A150057).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Zhoukou Normal University, East Wenchang Street, Chuanhui District, Zhoukou, 466001, P. R. China

    Wenping Zhu, Liyan Dai, Zengchen Liu, Weijie Yang, Chunxiang Zhao, Yanxia Li & Yahong Chen

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  1. Wenping Zhu
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  2. Liyan Dai
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  3. Zengchen Liu
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  4. Weijie Yang
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  5. Chunxiang Zhao
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  6. Yanxia Li
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  7. Yahong Chen
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Correspondence to Wenping Zhu.

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Zhu, W., Dai, L., Liu, Z. et al. Hairpin-shaped DNA Templated Copper Na nopar tides for Fluorescence Detection of Adenosine Triphosphate Based on Ligation-mediated Exonuclease Cleavage. ANAL. SCI. 33, 203–207 (2017). https://doi.org/10.2116/analsci.33.203

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  • DOI: https://doi.org/10.2116/analsci.33.203

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