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Identification of Proteinaceous Binders in Ancient Tripitaka by the Use of an Enzyme-linked Immunosorbent Assay

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Abstract

Proteinaceous materials, such as ovabumin and collagen, were commonly used as binding media, and as adhesives and protective coatings. However, the identification of ancient proteinaceous binders is a great challenge for archaeologists, due to their limited sample size, complex combinations of various ingredients and reduced availability of the binder during the process of protein degradation. In this paper, an enzyme-linked immunosorbent assay (ELISA) provides to be a particularly promising method for the detection of proteinaceous binding materials in ancient relics. The present work focused on the specific identification of proteins in archaeological binders, which was brushed on the Tripitaka. Two samples, the adhesion area (S1) and the ink area (S2), were tested by ELISA. The results showed that both S1 and S2 reacted positively when treated with an anti-collagen-I antibody. It proved the existence of proteinaceous binders in Ancient Tripitaka, and the percentage of collagen in S1 and S2 was 61.44 and 15.4%, respectively. Compared with other conventional techniques, ELISA has advantages of high specificity, sensitivity, rapidity and low cost, making it especially suitable for the protein detection in the archaeological field.

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Acknowledgments

Financial support was provided by the National Key Technology R&D Program (2013BAK08B08), Special Funds from the Administration of Cultural Heritage of Zhejiang Province (2014) and Science Foundation of Zhejiang Sci-Tec University (ZSTU) under Grant No. 13012141-Y

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Authors and Affiliations

  1. Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, 310018, China

    Yi Liu, Yi Li, Runxing Chang & Bing Wang

  2. Key Scientific Research Base of Textile Conservation, State Administration for Cultural Heritage, China National Silk Museum, Hangzhou, 310002, China

    Hailing Zheng & Yang Zhou

  3. Institute of Textile Conservation, Hangzhou, 310018, China

    Menglu Li & Zhiwen Hu

Authors
  1. Yi Liu
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  2. Yi Li
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  3. Runxing Chang
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  4. Hailing Zheng
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  5. Menglu Li
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  6. Zhiwen Hu
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  7. Yang Zhou
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  8. Bing Wang
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Correspondence to Yang Zhou or Bing Wang.

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Liu, Y., Li, Y., Chang, R. et al. Identification of Proteinaceous Binders in Ancient Tripitaka by the Use of an Enzyme-linked Immunosorbent Assay. ANAL. SCI. 32, 735–740 (2016). https://doi.org/10.2116/analsci.32.735

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  • DOI: https://doi.org/10.2116/analsci.32.735

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