Skip to main content
SpringerLink
Log in

Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

A lipophilic oxygen radical absorbance capacity (L-ORAC) assay is an evaluation of the antioxidant capacity of solutions of lipophilic compounds. The concentrations of fluorescein k]radical generator k]and Trolox standard solutions were optimized to improve the precision of the assay. An interlaboratory study using two antioxidant solutions and three food extracts as test samples conducted in accordance with harmonized protocol demonstrated satisfactory L-ORAC measurements; the intermediate precision relative standard deviations (RSRDint) ranged from 7.0 to 16.7% k]the reproducibility relative standard deviations (RSDR) ranged from 14.8 to 19.4% k]and the HorRat values ranged from 1.35 to 1.78.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. D.D. Rio, C. Agnoli, N. Pellegrini, J. Nutr., 2011, 141, 118.

    Article  PubMed  Google Scholar 

  2. Y. Wang, M. Yang, S.G. Lee, C.G. Davis, S.I. Koo, M.L. Fernandez, J.S. Volek, O.K. Chun, Eur. J. Nutr., 2014, 53, 1363.

    Article  CAS  PubMed  Google Scholar 

  3. B. Ou, M. Hampsch-Woodill, R.L. Prior, J. Agric. Food Chem., 2001, 49, 4619.

    Article  CAS  PubMed  Google Scholar 

  4. D. Huang, B. Ou, M. Hampsch-Woodill, J.A. Flanagan, R.L. Prior, J. Agric. Food Chem., 2002, 50, 4437.

    Article  CAS  PubMed  Google Scholar 

  5. B. Halliwell, Annu. Rev. Nutr., 1996, 16, 33.

    Article  CAS  PubMed  Google Scholar 

  6. D. Huang and B. Ou, J. Agric. Food Chem., 2002, 50, 1815.

    Article  Google Scholar 

  7. P.I. Pfitzner, P.I. Francz, H.K. Biesalski, Biochim. Biophys. Acta, 2000, 1474, 163.

    Article  CAS  PubMed  Google Scholar 

  8. X. Wu and G. Beecher, J. Agric. Food Chem., 2004, 52, 4026.

    Article  CAS  PubMed  Google Scholar 

  9. G.W. Burton and M.G. Traber, Annu. Rev. Nutr., 1990, 10, 357.

    Article  PubMed  Google Scholar 

  10. M.V. Eberhardt, K. Kobira, A.S. Keck, J.A. Juvik, E.H. Jeffery, J. Agric. Food Chem., 2005, 53, 7421.

    Article  CAS  PubMed  Google Scholar 

  11. J. Watanabe, T. Oki, J. Takebayashi, K. Yamasaki, Y. Takano-Ishikawa, A. Hino, A. Yasui, Anal. Sci., 2012, 28, 159.

    Article  CAS  PubMed  Google Scholar 

  12. J. Watanabe, T. Oki, J. Takebayashi, K. Yamasaki, Y. Takano-Ishikawa, A. Hino, A. Yasui, Biosci. Biotechnol. Biochem., 2013, 77, 857.

    Article  CAS  PubMed  Google Scholar 

  13. D. Jimenez-Alvarez, F. Giuffrida, F. Vanrobaeys, P. Golay, C. Cotting, A. Lardeau, B. Keely, J. Agric. Food Chem., 2008, 56, 3470.

    Article  CAS  PubMed  Google Scholar 

  14. W. Horwirz, Pure Appl. Chem., 1995, 67, 331.

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by a grant-in-aid “A Scheme to Revitalize Agriculture and Fisheries in Disaster Area through Deploying Highly Advanced Technology” from the Ministry of Agriculture, Forestry and Fisheries of Japan, and by JSPS KAKENHI Grant Number 26282200. We express our appreciation to the following collaborators for their participation in the study: Yuko Kino (Kagome Co.), Yuji Takahashi (Japan Food Research Laboratories), Shinji Owaki (Food Analysis Technology Center SUNATEC), Keiichi Negoro (Industrial Technology Center of Wakayama Prefecture), Ryo Nakagawa (Taiyo Kagaku Co.), Masao Sasaki (Thermo Scientific Co.), Toshihiko Shoji (National Institute of Fruit Tree Science, National Agriculture and Food Research Organization), Satoshi Shimizu (National Agricultural Research Center for Tohoku Region, National Agriculture and Food Research Organization), Yoichi Nokata (Western Region Agricultural Research Center, National Agriculture and Food Research Organization), Koji Ueda (Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization), Rie Kurata (Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization), Koji Ishiguro (Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization), Yumiko Konno (National Food Research Institute, National Agriculture and Food Research Organization), and Noriyuki Furutani (Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Center). We also express appreciation to Dr. Yasuhiro Suzuki (NARO Institute of Crop Science) for providing rice samples.

Author information

Authors and Affiliations

  1. National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, 305-8642, Japan

    Jun Watanabe, Hiroshi Yada, Manabu Wagaki, Yuko Takano-Ishikawa & Akemi Yasui

  2. National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Food Research Organization, 2421 Suya, Koshi, Kumamoto 861-1192, Japan

    Tomoyuki Oki

  3. National Institutes of Biomedical Innovation, Health and Nutrition, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku, Tokyo 162-8636, Japan

    Jun Takebayashi

Authors
  1. Jun Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tomoyuki Oki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jun Takebayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroshi Yada
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Manabu Wagaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuko Takano-Ishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Akemi Yasui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jun Watanabe.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Watanabe, J., Oki, T., Takebayashi, J. et al. Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts. ANAL. SCI. 32, 171–175 (2016). https://doi.org/10.2116/analsci.32.171

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.32.171

Keywords

Search

Navigation