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Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy

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Abstract

The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells' before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.

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Acknowledgements

We thank Thermo Fisher Scientific Inc., especially Dr. A. Makarov, for their support with scientific instruments, and Mrs. April Oga for assistance with writing this manuscript. This work was conducted under the RIKEN Pioneering Projects of “Single Cell Science” and “Biology of Symbiosis” and the fund for the Development of Advanced Measurement and Analysis Systems (SENTAN), by the Japan Agency for Medical Research and Development (AMED), and by the Japan Science and Technology Agency (JST) in 2013–2014.

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Authors and Affiliations

  1. Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai Suita, Osaka, 565-0874, Japan

    Ahmed Ali, Yasmine Abouleila, Sara Amer, Rie Furushima, Samy Emara & Tsutomu Masujima

  2. Faculty of Pharmacy, Misr International University, Km28 Ismailia Road, Cairo, Egypt

    Sebastien Equis

  3. Nanolive SA, Chemin de la Dent d'Oche 1a, Lausanne, 1024, Switzerland

    Yann Cotte

Authors
  1. Ahmed Ali
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  2. Yasmine Abouleila
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  3. Sara Amer
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  4. Rie Furushima
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  5. Samy Emara
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  6. Sebastien Equis
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  7. Yann Cotte
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  8. Tsutomu Masujima
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Correspondence to Tsutomu Masujima.

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Ali, A., Abouleila, Y., Amer, S. et al. Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy. ANAL. SCI. 32, 125–127 (2016). https://doi.org/10.2116/analsci.32.125

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  • DOI: https://doi.org/10.2116/analsci.32.125

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