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Patterned Co-culture of Live Cells on a Microchip by Photocrosslinking with Benzophenone

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Abstract

The patterned coculture of different types of living cells in a microfluidic device is crucial for the analysis of cellular interactions and cell-cell communication. In the present study, cell patterning was achieved by photocrosslinking benzophenone derivatives in a microfluidic channel. Optimization of UV irradiation conditions enabled successful fixation of live cells. In addition, patterning and co-culture of non-adherent K562 cells and adherent RF-6A cells was achieved by successive rounds of patterning. The present approach is expected to be useful for the development of in vitro methods for studying cell signaling.

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Acknowledgments

This work was supported in part by the NEDO Industrial Technology Research Assistance Project, Shimadzu Science Foundation, Asahi Glass Foundation, and a Grant-in-Aid for Scientific Research (JSPS, KAKENHI; Grant Numbers 15K15199, 24350035, and 15H03823).

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, School of Science and Technology, Gunma University, Kiryu, Gunma, 376-8515, Japan

    Kiichi Sato, Sayaka Kikuchi & Kin-ichi Tsunoda

  2. Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo, 112-8681, Japan

    Eri Yoshida, Reina Ishii, Naoki Sasaki & Kae Sato

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  1. Kiichi Sato
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  2. Sayaka Kikuchi
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  3. Eri Yoshida
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  4. Reina Ishii
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  5. Naoki Sasaki
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  6. Kin-ichi Tsunoda
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  7. Kae Sato
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Correspondence to Kiichi Sato.

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Sato, K., Kikuchi, S., Yoshida, E. et al. Patterned Co-culture of Live Cells on a Microchip by Photocrosslinking with Benzophenone. ANAL. SCI. 32, 113–116 (2016). https://doi.org/10.2116/analsci.32.113

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  • DOI: https://doi.org/10.2116/analsci.32.113

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