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A Miniaturized Stepwise Injection Spectrophotometric Analyzer

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Abstract

A novel micro-stepwise injection analyzer (μSWIA) has been developed for the automation and miniaturization of spectrophotometric analysis. The main unit of this device is a mixing chamber (MC) connected to the atmosphere. This part of the μSWIA provides rapid and effective homogenization of the reaction mixture components and completion of the reaction by means of gas bubbling. The μSWIA contained a rectangular labyrinth channel designed in way allowing one to eliminate bubbles by moving a solution from the MC to an optical channel. The light-emitting diode (LED) was used as a light emitter and the analytical signal was measured by a portable spectrophotometer. Fluid movement was attained via the use of a computer-controlled syringe pump. The μSWIA was successfully used for the spectrophotometric determination of cysteine in biologically active supplements and fodder by using 18-molybdo-2-phosphate heteropoly anion (18-MPA) as the reagent.

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Acknowledgments

We gratefully acknowledge financial support of the Russian Foundation for Basic Research (Grant No. 13-03-00031). This work was supported by the Ministry of Education and Science of the Russian Federation in form of Scholarship of the Russian President (Order No. 539). Scientific research was performed at the Center for chemical analysis and materials research of St. Petersburg State University.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg State University, pr. Universitetskij 26, Saint-Petersburg, 19850, Russia

    Anastasiia Petrova, Andrey Bulatov & Leonid Moskvin

  2. Department of Analytical Chemistry, Institute of Chemistry, Oles Honchar Dnipropetrovsk National University, Gagarina 72, Dnipropetrovsk, 49010, Ukraine

    Andrey Vishnikin

  3. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi, Fukuoka, 819-0395, Japan

    Ryoichi Ishimatsu, Koji Nakano & Toshihiko Imato

Authors
  1. Anastasiia Petrova
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  2. Andrey Bulatov
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  3. Andrey Vishnikin
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  4. Leonid Moskvin
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  5. Ryoichi Ishimatsu
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  6. Koji Nakano
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  7. Toshihiko Imato
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Correspondence to Anastasiia Petrova.

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Petrova, A., Bulatov, A., Vishnikin, A. et al. A Miniaturized Stepwise Injection Spectrophotometric Analyzer. ANAL. SCI. 31, 529–533 (2015). https://doi.org/10.2116/analsci.31.529

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  • DOI: https://doi.org/10.2116/analsci.31.529

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