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Application of a Novel Near Infrared-Fluorescence Giant Vesicle-and Polymerasome-based Tissue Marker for Endoscopic and Laparoscopic Navigation

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Abstract

In this study, we describe the development of a novel tissue marker that can be injected from within the digestive tract by using an endoscopic instrument, and visualized using near-infrared (NIR) fluorescence imaging. The marker was prepared in three steps, (i) mixing NIR-fluorescent indocyanine green (ICG) with giant vesicles (GVs) of lecithin, (ii) suspending the ICG-containing giant vesicles (ICG-GV) in an oil phase dissolving polyglycerol-polyricinoleate (PGPR), and (iii) centrifugation of the suspension layered on a buffered solution to obtain a giant polymer vesicle (polymerasome) containing ICG-GV. We injected the tissue marker into the inner gastric surface of an anesthetized pig using an endoscopic syringe, and observed the injection site using a fluorescence laparoscopic camera. The diameter of the spot blur was approximately 2 cm over a 5-h period, demonstrating the utility of this procedure as a tissue marker for tumor marking, and suggesting its potential for assisting navigation during surgical procedures.

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Authors and Affiliations

  1. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan

    Hirosuke Hatayama, Taro Toyota, Tomonori Nomoto & Masanori Fujinami

  2. Department of Bioinformatics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo, Chiba, 260-8670, Japan

    Taro Toyota

  3. Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan

    Taro Toyota

  4. Center for Frontier Medical Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan

    Hideki Hayashi

  5. Department of Frontier Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo, Chiba, 260-8670, Japan

    Hideki Hayashi

Authors
  1. Hirosuke Hatayama
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  2. Taro Toyota
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  3. Hideki Hayashi
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  4. Tomonori Nomoto
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  5. Masanori Fujinami
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Correspondence to Masanori Fujinami.

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Hatayama, H., Toyota, T., Hayashi, H. et al. Application of a Novel Near Infrared-Fluorescence Giant Vesicle-and Polymerasome-based Tissue Marker for Endoscopic and Laparoscopic Navigation. ANAL. SCI. 30, 225–230 (2014). https://doi.org/10.2116/analsci.30.225

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  • DOI: https://doi.org/10.2116/analsci.30.225

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