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Two-Photon Photoionization Limits of Detection of Polycyclic Aromatic Hydrocarbons in Reversed Phase Liquid Chromatography

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Abstract

Two-photon photoionization detection was used to detect 28 polycyclic aromatic hydrocarbons (PAHs) separated by reversed phase liquid chromatography. A windowless flow cell and a nitrogen laser were used to obtain the photoionization signals. The analytical merit of the technique was illustrated by the determination of the limits of detection (LOD) for the 28 PAHs. LODs as low as 50 and 70 pg injected were obtained for anthracene and pyrene, respectively. The importance of the chromatographic efficiency is discussed. The dramatic influence of the mobile phase conductivity, which is mainly responsible for the leakage currents, is shown. The lower the leakage current and the solvent polarity, the better the two-photon photoionization detection.

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References

  1. J. Jacob, W. Karcher and P. J. Wagstaffe, Fresenius’ Z. Anal Chem., 317, 101 (1984).

    Article  CAS  Google Scholar 

  2. J. P. Lowe and B. D. Silverman, Ace. Chem. Res., 17, 332 (1984).

    Article  CAS  Google Scholar 

  3. K. S. Chin, K. Bieman, K. Krishnan and S. L. Hill, Anal. Chem., 56, 1610 (1984).

    Article  Google Scholar 

  4. K. Ogan, E. Katz and W. Slavin, Anal. Chem., 51, 1315 (1979).

    Article  CAS  Google Scholar 

  5. T. Nielsen, J. Chromatogr., 170, 147 (1979).

    Article  CAS  Google Scholar 

  6. P. A. Peaden, M. L. Lee, Y. Hirata and M. Novotny, Anal. Chem., 52, 2268 (1980).

    Article  CAS  Google Scholar 

  7. W. E. May and S. A. Wise, Anal. Chem., 56, 225 (1984).

    Article  CAS  Google Scholar 

  8. S. C. Ruckmick and R. J. Hurtubise, J. Chromatogr., 321, 343 (1985).

    Article  CAS  Google Scholar 

  9. D. T. Rossi, D. J. Desilets and H. L. Pardue, Anal Chim. Acta, 161, 191 (1984).

    Article  CAS  Google Scholar 

  10. H. Y. Tong and F. W. Karasek, Anal. Chem., 56, 2129 (1984).

    Article  CAS  Google Scholar 

  11. K. Tanabe, M. Glick and J. D. Winefordner, Anal. Chem., 59, 1125 (1987).

    Article  CAS  Google Scholar 

  12. A. Berthod, K. P. Li, T. Yu and J. D. Winefordner, Anal. Chem., 59, 1485 (1987).

    Article  CAS  Google Scholar 

  13. E. P. C. Lai, S. Y. Su, E. Voigtman and J. D. Winefordner, Chromatographia, 15, 645 (1982).

    Article  CAS  Google Scholar 

  14. E. Voigtman, A. Jurgensen and J. D. Winefordner, Anal. Chem., 53, 1921 (1981).

    Article  CAS  Google Scholar 

  15. E. Voigtman and J. D. Winefordner, Anal Chem., 54, 1834 (1982).

    Article  CAS  Google Scholar 

  16. S. Yamada and T. Ogawa, Prog. Analyt. Spectrosc., 9, 429 (1986).

    CAS  Google Scholar 

  17. S. Yamada, A. Hino and T. Ogawa, Anal Chim. Acta., 156, 273 (1984); Anal. Chem., 55, 1914 (1983).

    Article  CAS  Google Scholar 

  18. E. Voigtman and J. D. Winefordner, J. Liq. Chromatogr., 5, 2113 (1982).

    Article  CAS  Google Scholar 

  19. E. Voigtman and J. D. Winefordner, Talanta, 30, 75 (1983).

    Article  CAS  Google Scholar 

  20. L. R. Snyder, J. J. Kirkland in “Introduction to Modern Liquid Chromatography”, p. 560, Wiley-Interscience, New York (1979).

    Google Scholar 

  21. J. L. Franklin, J. G. Dilliard, H. M. Rosenstock, J. T. Herron, K. Draxl and F. H. Field, “Ionization Potentials, Appearance Potentials, and Heats of Formation of Gaseous Positive Ions”, NSRDS-NBS 26, National Bureau of Standards, Washington D.C., 1969.

    Book  Google Scholar 

  22. L. Onsager, Phys. Rev., 15, 554 (1938).

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Florida, Gainesville, FL, 32611, USA

    Alain Berthod, Tony Mellone, Edward Voigtman & J. D. Winefordner

  2. On leave from Laboratoire de Sciences Analytigues, Université de Lyon 1, 69622, Villeurbanne Cedex, France

    Alain Berthod

  3. Chemistry Department, Lederle Graduate Research Center, University of Massachussetts, Amherst, MA, 01003, USA

    Edward Voigtman

Authors
  1. Alain Berthod
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  2. Tony Mellone
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  3. Edward Voigtman
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  4. J. D. Winefordner
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Additional information

This research was supported by NIH-GM 11373–23.

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Berthod, A., Mellone, T., Voigtman, E. et al. Two-Photon Photoionization Limits of Detection of Polycyclic Aromatic Hydrocarbons in Reversed Phase Liquid Chromatography. ANAL. SCI. 3, 405–411 (1987). https://doi.org/10.2116/analsci.3.405

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  • DOI: https://doi.org/10.2116/analsci.3.405

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