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Simultaneous Purification of Carboxypeptidase A, Carboxypeptidase B and Chymotrypsin C from Autolyzed Porcine Pancreas Glands

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Abstract

Carboxypeptidases A and B and chymotrypsin C from outolyzed porcine pancreas glands were simultaneously purified by extraction of acetone powder with water, ammonium sulfate precipitation, and chromatography on DEAE-cellulose. Then carboxypeptidases A and B were freed from contaminated endopeptidase such as elastase and chymotrypsin C by affinity chromatography on d-phenylalanine-CH-Sepharose 4B and d-arginine-CH-Sepharose 4B, respectively. Chymotrypsin C was freed from carboxypeptidases A and B by passage through d-phenylalanine-CH-Sepharose 4B. These carboxypeptidases A and B can be used for carboxyl-terminal analysis of peptides without prior treatment with diisopropyl phosphorofluoridate to inactivate contaminated pancreatic endopeptidases.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Chiba University, Yayoi-cho, Chiba, 260, Japan

    Kuniko Akama & Tohru Tobita

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  1. Kuniko Akama
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  2. Tohru Tobita
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Akama, K., Tobita, T. Simultaneous Purification of Carboxypeptidase A, Carboxypeptidase B and Chymotrypsin C from Autolyzed Porcine Pancreas Glands. ANAL. SCI. 3, 167–170 (1987). https://doi.org/10.2116/analsci.3.167

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  • DOI: https://doi.org/10.2116/analsci.3.167

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