Skip to main content
SpringerLink
Log in

Graphene-modified Interdigitated Array Electrode: Fabrication, Characterization, and Electrochemical Immunoassay Application

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

We have developed a new procedure for fabricating interdigitated array gold electrodes (Au-IDA) modified with reduced graphene oxide (rGO). In this procedure, we coated the gold surface of the micrometer order electrodes with graphene oxide (GO) prior to the reduction and the lift-off processes to avoid short-circuiting the pair of electrodes by conductive rGO flakes after the reduction. We then studied the basic electrochemical activity of the prepared electrodes, rGO/Au-IDA, mainly on p-aminophenol (pAP), because pAP is a good probe for an electrochemical immunoassay. The voltammograms showed that denser rGO provides better electrode reactivity for pAP. We confirmed that redox cycling between the anode and cathode at the rGO/Au-IDA was established, which yields more sensitive detection than with a single electrode. As one application of the electrochemical immunoassay using the rGO/Au-IDA, we demonstrated the quantitative detection of cortisol, a stress marker, at levels found in human saliva.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Y. Shao, J. Wang, H. Wu, J. Liu, I. A. Aksay, and Y. Lin, Electroanalysis, 2010, 22, 1027.

    Article  CAS  Google Scholar 

  2. D. A. C. Brownson and C. E. Banks, Analyst, 2010, 135, 2768.

    Article  CAS  PubMed  Google Scholar 

  3. D. Li, M. B. Müller, S. Gilje, R. B. Kaner, and G. G. Wallace, Nat. Nanotechnol., 2008, 3, 101.

    Article  CAS  PubMed  Google Scholar 

  4. S. Alwarappan, A. Erdem, C. Liu, and C.-A. Li, J. Phys. Chem. C, 2009, 113, 8853.

    Article  CAS  Google Scholar 

  5. M. Zhou, Y. Zhai, and S. Dong, Anal. Chem., 2009, 81, 5603.

    Article  CAS  PubMed  Google Scholar 

  6. J. Zhu, X. Chen, and W. Yang, Sens. Actuators, B, 2010, 150, 564.

    Article  CAS  Google Scholar 

  7. M. Du, T. Yang, and K. Jiao, J. Mater. Chem., 2010, 20, 9253.

    Article  CAS  Google Scholar 

  8. W.-J. Lin, C.-S. Liao, J.-H. Jhang, and Y.-C. Tsai, Electrochem. Commun., 2009, 11, 2153.

    Article  CAS  Google Scholar 

  9. S. Yang, B. Xu, J. Zhang, X. Huang, J. Ye, and C. Yu, J. Phys. Chem. C, 2010, 114, 4389.

    Article  CAS  Google Scholar 

  10. X. Xie, K. Zhao, X. Xu, W. Zhao, S. Liu, Z. Zhu, M. Li, Z. Shi, and Y. Shao, J. Phys. Chem. C, 2010, 114, 14243.

    Article  CAS  Google Scholar 

  11. F. Li, M. Xue, X. Ma, M. Zhang, and T. Cao, Anal. Chem., 2011, 83, 6426.

    Article  CAS  PubMed  Google Scholar 

  12. R. S. Day and C. R. Raj, J. Phys. Chem. C, 2010, 114, 21427.

    Article  Google Scholar 

  13. D.-W. Wang, I. R. Gentle, and G. Q. Lu, Electrochem. Commun., 2010, 12, 1423.

    Article  CAS  Google Scholar 

  14. K. Aoki, M. Morita, O. Niwa, and H. Tabei, J. Electroanal. Chem., 1988, 256, 269.

    Article  CAS  Google Scholar 

  15. H. T. Tang, C. E. Lunte, H. B. Halsall, and W. R. Heineman, Anal. Chim. Acta, 1988, 214, 187.

    Article  CAS  Google Scholar 

  16. L. Anfossi, C. Tozzi, C. Giovannoli, C. Baggiani, and G. Giraudi, Anal. Chim. Acta, 2002, 468, 315.

    Article  CAS  Google Scholar 

  17. W. S. Hummers and R. E. Offeman, J. Am. Chem. Soc., 1958, 80, 1339.

    Article  CAS  Google Scholar 

  18. S. Uhm, N. H. Tuyen, and J. Lee, Electrochem. Commun., 2011, 13, 677.

    Article  CAS  Google Scholar 

  19. S. Stankovich, D. A. Dikin, R. D. Piner, K. A. Kohlhaas, A. Kleinhammes, Y. Jia, Y. Wu, S. T. Nguyen, and R. S. Ruoff, Carbon, 2007, 45, 1558.

    Article  CAS  Google Scholar 

  20. A. Ueda, D. Kato, R. Kurita, T. Kamata, H. Inokuchi, S. Umemura, S. Hirono, and O. Niwa, J. Am. Chem. Soc., 2011, 133, 4840.

    Article  CAS  PubMed  Google Scholar 

  21. K. Hayashi, J. Takahashi, T. Horiuchi, Y. Iwasaki, and T. Haga, J. Electrochem. Soc., 2008, 155, J240.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. NTT Microsystem Integration Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa, 243-0198, Japan

    Yuko Ueno, Katsuyoshi Hayashi & Emi Tamechika

  2. NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa, 243-0198, Japan

    Kazuaki Furukawa, Makoto Takamura & Hiroki Hibino

Authors
  1. Yuko Ueno
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kazuaki Furukawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Katsuyoshi Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Makoto Takamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hiroki Hibino
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Emi Tamechika
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuko Ueno.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ueno, Y., Furukawa, K., Hayashi, K. et al. Graphene-modified Interdigitated Array Electrode: Fabrication, Characterization, and Electrochemical Immunoassay Application. ANAL. SCI. 29, 55–60 (2013). https://doi.org/10.2116/analsci.29.55

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.29.55

Search

Navigation