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Determination of Acid Dissociation Constant of Degradable Tetrabromophenolphthalein Ethyl Ester by Capillary Zone Electrophoresis

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Abstract

An acid dissociation constant of tetrabromophenolphthalein ethyl ester (TBPE) was determined through the measurement of electrophoretic mobility by capillary zone electrophoresis (CZE). Although TBPE is degradable in acidic pH region and it gradually degraded at pH conditions around and below its pKa values in the time scale of the CZE measurement, equilibrium species of interest were detected as a peak-shaped signal with tailing of the degraded species. Changes in electrophoretic mobility of the equilibrium species of TBPE were analyzed at its detectable pH range in the presence of phenol red as a mobility standard. An acid dissociation constant of 3.47 ± 0.06 (pKa, I = 0.01, 25°C, standard error) was determined for TBPE.

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  1. Toshio Takayanagi

    Present address: Present address: Department of Life System, Institute of Technology and Science, The University of Tokushima, 2-1 Minamijyousanjimacho, 770-8506, Tokushima, Japan

Authors and Affiliations

  1. Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, 700-8530, Kita, Okayama, Japan

    Toshio Takayanagi, Ayumi Tabara & Takashi Kaneta

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  1. Toshio Takayanagi
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  2. Ayumi Tabara
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  3. Takashi Kaneta
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Correspondence to Toshio Takayanagi.

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Takayanagi, T., Tabara, A. & Kaneta, T. Determination of Acid Dissociation Constant of Degradable Tetrabromophenolphthalein Ethyl Ester by Capillary Zone Electrophoresis. ANAL. SCI. 29, 547–552 (2013). https://doi.org/10.2116/analsci.29.547

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  • DOI: https://doi.org/10.2116/analsci.29.547

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