Abstract
A solution of polyhexamethylene biguanide hydrochloride (PHMB-HCl) was titrated with a standard solution of potassium poly(vinyl sulfate) (PVSK) using crystal violet (CV) as an photometric indicator cation. The end point was detected by a sharp absorbance change due to an abrupt decrease in the concentration of CV. A linear relationship between the concentration of PHMB-HCl and the end-point volume of the titrant existed in the concentration range from 2 to 10 × 10−6 eq mol L−1. Back-titration was based on adding an excess amount of PVSK to a sample solution containing CV, which was titrated with a standard solution of poly(diallyldimethylammonium chloride) (PDADMAC). The calibration curve of the PHMB-HCl concentration to the end point volume of the titrant was also linear in the concentration range from 2 to 8 × 10−6 eq mol L−1. Both photometric titrations were applied to the determination of PHMB-HCl in a few contact-lens detergents. Back-titration showed a clear end point, but direct titration showed an unclear end point. The results of the back-titration of PHMB-HCl were compared with the content registered in its labels.
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Masadome, T., Miyanishi, T., Watanabe, K. et al. Determination of Polyhexamethylene Biguanide Hydrochloride Using Photometric Colloidal Titration with Crystal Violet as a Color Indicator. ANAL. SCI. 27, 817–821 (2011). https://doi.org/10.2116/analsci.27.817
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DOI: https://doi.org/10.2116/analsci.27.817